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PMID:14715936

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Citation

Sadakata, T, Mizoguchi, A, Sato, Y, Katoh-Semba, R, Fukuda, M, Mikoshiba, K and Furuichi, T (2004) The secretory granule-associated protein CAPS2 regulates neurotrophin release and cell survival. J. Neurosci. 24:43-52

Abstract

Neurotrophins are key modulators of various neuronal functions, including differentiation, survival, and synaptic plasticity, but the molecules that regulate their secretion are poorly understood. We isolated a clone that is predominantly expressed in granule cells of postnatally developing mouse cerebellum, which turned out to be a paralog of CAPS (Ca2+-dependent activator protein for secretion), and named CAPS2. CAPS2 is enriched on vesicular structures of presynaptic parallel fiber terminals of granule cells connecting postsynaptic spines of Purkinje cell dendrites. Vesicle factions affinity-purified by the CAPS2 antibody from mouse cerebella contained significant amounts of neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), and chromogranin B but not marker proteins for synaptic vesicle synaptophysin and synaptotagmin. In cerebellar primary cultures, punctate CAPS2 immunoreactivities are primarily colocalized with those of NT-3 and BDNF and near those of a postsynaptic marker, postsynaptic density-95, around dendritic arborization of Purkinje cells. Exogenously expressed CAPS2 enhanced release of exogenous NT-3 and BDNF from PC12 cells and endogenous NT-3 from cultured granule cells in a depolarization-dependent manner. Moreover, the overexpression of CAPS2 in granule cells promotes the survival of Purkinje cells in cerebellar cultures. Thus, we suggest that CAPS2 mediates the depolarization-dependent release of NT-3 and BDNF from granule cells, leading to regulation in cell differentiation and survival during cerebellar development.

Links

PubMed Online version:10.1523/JNEUROSCI.2528-03.2004

Keywords

Amino Acid Sequence; Animals; Brain/anatomy & histology; Brain/growth & development; Brain/metabolism; Calcium-Binding Proteins/analysis; Calcium-Binding Proteins/genetics; Calcium-Binding Proteins/physiology; Cell Survival; Cells, Cultured; Cerebellum/cytology; Cerebellum/growth & development; Cerebellum/metabolism; Exocytosis; Humans; In Situ Hybridization; Mice; Molecular Sequence Data; Nerve Growth Factors/analysis; Nerve Growth Factors/metabolism; Nerve Tissue Proteins/analysis; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/physiology; Neurons/chemistry; Neurons/cytology; Neurons/metabolism; PC12 Cells; Presynaptic Terminals/chemistry; Purkinje Cells/cytology; Purkinje Cells/metabolism; RNA, Messenger/analysis; Rats; Secretory Vesicles/chemistry; Sequence Alignment

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:NTF3

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000121)

Seeded From UniProt

complete

MOUSE:BDNF

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000121)

Seeded From UniProt

complete

MOUSE:CAPS2

located_in

GO:0045211: postsynaptic membrane

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:35718)

Seeded From UniProt

complete

MOUSE:CAPS2

located_in

GO:0042734: presynaptic membrane

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:35718)

Seeded From UniProt

complete

MOUSE:CAPS2

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:35718)

Seeded From UniProt

complete

MOUSE:CAPS2

acts_upstream_of_or_within

GO:0045921: positive regulation of exocytosis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CAPS2

part_of

GO:0098793: presynapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:CAPS2

is_active_in

GO:0098793: presynapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:CAPS2

part_of

GO:0098688: parallel fiber to Purkinje cell synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:CAPS2

is_active_in

GO:0098688: parallel fiber to Purkinje cell synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:BDNF

part_of

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(CL:0000121)

Seeded From UniProt

complete

See also

References

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