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PMID:14714568

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Citation

Greig, AV, James, SE, McGrouther, DA, Terenghi, G and Burnstock, G (2003) Purinergic receptor expression in the regeneration epidermis in a rat model of normal and delayed wound healing. Exp. Dermatol. 12:860-71

Abstract

This study investigated changes in the protein expression of purinergic receptors in the regenerating rat epidermis during normal wound healing, in denervated wounds, and in denervated wounds treated with nerve growth factor (NGF), where wound healing rates are normalized. Excisional wounds were placed within denervated, pedicled, oblique, groin skin flaps, and in the contralateral abdomen to act as a control site. Six rats had NGF-treated wounds and six had untreated wounds. Tissue was harvested at day four after wounding. The re-epithelializing wound edges were analyzed immunohistochemically for P2X(5), P2X(7), P2Y(1) and P2Y(2) receptors, and immunostaining of keratinocytes was quantified using optical densitometry. In normal rat epidermis, P2Y(1) and P2Y(2) receptors were found in the basal layer where keratinocytes proliferate; P2X(5) receptors were associated with proliferating and differentiating epidermal keratinocytes in basal and suprabasal layers; P2X(7) receptors were associated with terminally differentiated keratinocytes in the stratum corneum. In the regenerating epidermis of denervated wounds, P2Y(1) receptor protein expression was significantly increased in keratinocytes (P<0.001) but P2Y(1) receptors (P<0.001) compared with untreated denervated wounds. In innervated wounds, NGF treatment enhanced expression in keratinocytes. P2X(5) (P>0.001) and P2Y(1) receptor protein (P<0.001) expression in keratinocytes. P2X(7) receptors were absent in all experimental wound healing preparations. P2X(5), P2X(7), P2Y(1) and P2Y(2) receptor protein expression in the regenerating epidermis was altered both during wound healing and also by NGF treatment. Possible roles for purinergic signalling and its relation to NGF in wound healing are discussed.

Links

PubMed

Keywords

Animals; Cell Division; Densitometry; Epidermis/metabolism; Epidermis/pathology; Epidermis/physiology; Immunohistochemistry; Keratinocytes/cytology; Keratinocytes/metabolism; Nerve Growth Factor/metabolism; Nerve Growth Factors; Phenotype; Rats; Receptors, Purinergic/biosynthesis; Receptors, Purinergic P2/biosynthesis; Receptors, Purinergic P2/metabolism; Receptors, Purinergic P2X5; Receptors, Purinergic P2X7; Receptors, Purinergic P2Y1; Receptors, Purinergic P2Y2; Regeneration; Skin/pathology; Time Factors; Wound Healing

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:P2RY1

involved_in

GO:0042060: wound healing

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:P2RY1

involved_in

GO:0070848: response to growth factor

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:P2RY2

involved_in

GO:0042060: wound healing

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:P2RY2

involved_in

GO:0070848: response to growth factor

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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