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PMID:14697667

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Citation

Barbato, C, Corbi, N, Canu, N, Fanciulli, M, Serafino, A, Ciotti, M, Libri, V, Bruno, T, Amadoro, G, De Angelis, R, Calissano, P and Passananti, C (2003) Rb binding protein Che-1 interacts with Tau in cerebellar granule neurons. Modulation during neuronal apoptosis. Mol. Cell. Neurosci. 24:1038-50

Abstract

Che-1 is a recently identified human Rb binding protein that inhibits the Rb growth-suppressing function and regulates cell proliferation. Che-1 contacts the Rb and competes with HDAC1 for Rb-binding site, removing HDAC1 from the Rb/E2F cell cycle-regulated promoters. We have investigated the expression of Che-1 in neuronal cells and we showed that Che-1 directly interacts with Tau. Tau is a microtubule-associated protein involved in the assembly and stabilization of neuronal microtubules network that plays a crucial role modulating neuronal morphogenesis, axonal shape, and transport. In rat cerebellar granule neurons (CGNs) Che-1 partially colocalizes with Tau in the cytoplasm. Che-1 binds the amino-terminal region of Tau protein, which is not involved in microtubule interactions. Tau and Che-1 endogenous proteins coimmunoprecipitate from CGNs cellular lysates. In addition, Che-1/Tau interaction was demonstrated both in overexpressing COS-7 cells and CGNs by FRET analysis. Finally, we observed that Tau/Che-1 interaction is modulated during neuronal apoptosis.

Links

PubMed

Keywords

Animals; Apoptosis/physiology; Cercopithecus aethiops; Cerebellum/enzymology; Cerebellum/metabolism; Dogs; Humans; Mutation; Neurons/enzymology; Neurons/metabolism; Protein Binding/physiology; Protein Kinases/metabolism; Rats; Rats, Wistar; Retinoblastoma Protein/metabolism; tau Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:TAU

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:620685

F

Seeded From UniProt

complete

RAT:AATF

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:AATF

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:AATF

located_in

GO:0005794: Golgi apparatus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:AATF

enables

GO:0048156: tau protein binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:69329

F

Seeded From UniProt

complete


See also

References

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