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PMID:1468562

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Citation

Jüngst, A and Zumft, WG (1992) Interdependence of respiratory NO reduction and nitrite reduction revealed by mutagenesis of nirQ, a novel gene in the denitrification gene cluster of Pseudomonas stutzeri. FEBS Lett. 314:308-14

Abstract

An open reading frame, designated nirQ, was identified upstream of nirS, the structural gene for the respiratory nitrite reductase of Pseudomonas stutzeri ZoBell. Its derived gene product (275 amino acids, M(r) = 30,554) shows similarity to the NtrC protein family of transcriptional activators. Deletion-replacement mutagenesis of the nirQ gene resulted in the simultaneous loss of nitrite reduction and NO reduction in vivo. However, both reductases were still synthesized, with only nitrite reductase being active in vitro. NO reductase was overproduced by a factor of about 2. Our results indicate that the systems for nitrite reduction and NO reduction are functionally coupled.

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Keywords

Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Cloning, Molecular; DNA, Bacterial; Genes, Bacterial; Molecular Sequence Data; Multigene Family; Mutagenesis; Nitric Oxide/metabolism; Nitrites/metabolism; Oxidation-Reduction; Pseudomonas/genetics; Pseudomonas/metabolism; Restriction Mapping; Sequence Homology, Amino Acid; Trans-Activators

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

PSEST:NIRQ

GO:0019333: denitrification pathway

ECO:0000315:

P

Table 1: This table shows that NirQ is involved in denitrification. Researchers manipulated the NirQ gene. The results from table 1 showed that the mutant phenotype of NirQ yielded very low nitrite-reducatse activity and had no nitric oxide reduction activity.

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References

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