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PMID:14662900

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Citation

Lee, I, Wang, L, Wells, AD, Ye, Q, Han, R, Dorf, ME, Kuziel, WA, Rollins, BJ, Chen, L and Hancock, WW (2003) Blocking the monocyte chemoattractant protein-1/CCR2 chemokine pathway induces permanent survival of islet allografts through a programmed death-1 ligand-1-dependent mechanism. J. Immunol. 171:6929-35

Abstract

Islet allografts are subject to rapid rejection through host cellular immune responses involving mononuclear cell recruitment and tissue injury. Interruption of leukocyte recruitment through chemokine receptor targeting is of therapeutic benefit in various experimental models, but little is known about the contribution of chemokine pathways to islet allograft rejection. We found that murine islets produce monocyte chemoattractant protein-1 (MCP-1; CCL2) in vitro and that islet allograft rejection was associated with intragraft expression of MCP-1 and its receptor, CCR2. We therefore investigated whether MCP-1 and CCR2 are required for the rejection of fully MHC-disparate islet allografts. Wild-type mice treated with blocking anti-MCP-1 mAb plus a brief, subtherapeutic course of rapamycin had long-term islet allograft survival, in contrast to the effect of treatment with either mAb or rapamycin alone. CCR2(-/-) mice treated with rapamycin also maintained islet allografts long-term. Both MCP/CCR2- and rapamycin-sensitive signals were required for maximal proliferation of alloreactive T cells, suggesting that MCP-1/CCR2 induce rejection by promoting alloreactive T cell clonal expansion and homing and migration. Prolonged islet allograft survival achieved by blockade of the MCP-1/CCR2 pathway plus rapamycin therapy was accompanied by a mononuclear cell infiltrate expressing the inhibitory receptor, programmed death-1 (PD-1), and its ligand (PD-L1, B7-H1), and prolongation of islet allograft survival was abrogated by anti-PD-L1 mAb therapy. These data show that the blockade of MCP-1 binding to CCR2 in conjunction with subtherapeutic immunosuppression can have profound effects on islet allograft survival and implicate the expression of the PD-1/PD-L1 pathway in the regulation of physiologic responses in vivo.

Links

PubMed

Keywords

Animals; Antibodies, Monoclonal/administration & dosage; Antigens, CD274; Antigens, CD80; Antigens, Surface/physiology; Apoptosis Regulatory Proteins; Blood Proteins/antagonists & inhibitors; Blood Proteins/immunology; Blood Proteins/metabolism; Blood Proteins/physiology; Cell Differentiation/genetics; Cell Differentiation/immunology; Cell Division/genetics; Cell Division/immunology; Chemokine CCL2/antagonists & inhibitors; Chemokine CCL2/biosynthesis; Chemokine CCL2/physiology; Clone Cells; Dose-Response Relationship, Immunologic; Female; Graft Enhancement, Immunologic/methods; Graft Rejection/immunology; Graft Survival/drug effects; Graft Survival/genetics; Graft Survival/immunology; Islets of Langerhans Transplantation/immunology; Islets of Langerhans Transplantation/pathology; Ligands; Membrane Glycoproteins; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Knockout; Organ Culture Techniques; Peptides/antagonists & inhibitors; Peptides/immunology; Peptides/metabolism; Peptides/physiology; Programmed Cell Death 1 Receptor; Proteins/metabolism; Proteins/physiology; Receptors, CCR2; Receptors, Chemokine/antagonists & inhibitors; Receptors, Chemokine/physiology; Signal Transduction/drug effects; Signal Transduction/genetics; Signal Transduction/immunology; Sirolimus/administration & dosage; Sirolimus/therapeutic use; T-Lymphocyte Subsets/cytology; T-Lymphocyte Subsets/immunology; Up-Regulation/drug effects; Up-Regulation/genetics; Up-Regulation/immunology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CCL2

involved_in

GO:0050870: positive regulation of T cell activation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CCL2

involved_in

GO:0071356: cellular response to tumor necrosis factor

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CCL2

involved_in

GO:0071347: cellular response to interleukin-1

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CCL2

involved_in

GO:0071346: cellular response to interferon-gamma

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CCL2

involved_in

GO:0071222: cellular response to lipopolysaccharide

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CCR2

involved_in

GO:0002829: negative regulation of type 2 immune response

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CCR2

involved_in

GO:0032743: positive regulation of interleukin-2 production

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CCR2

involved_in

GO:0032729: positive regulation of interferon-gamma production

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CCR2

involved_in

GO:2000451: positive regulation of CD8-positive, alpha-beta T cell extravasation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CCR2

involved_in

GO:0050870: positive regulation of T cell activation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CCR2

involved_in

GO:0046641: positive regulation of alpha-beta T cell proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CCR2

involved_in

GO:0045627: positive regulation of T-helper 1 cell differentiation

ECO:0000305: curator inference used in manual assertion

GO:0032729

P

Seeded From UniProt

complete

MOUSE:CCR2

involved_in

GO:0043310: negative regulation of eosinophil degranulation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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