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PMID:14645293

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Citation

Wozniak, DJ, Sprinkle, AB and Baynham, PJ (2003) Control of Pseudomonas aeruginosa algZ expression by the alternative sigma factor AlgT. J. Bacteriol. 185:7297-300

Abstract

AlgZ controls Pseudomonas aeruginosa alginate synthesis by activating algD, yet algZ expression is not detectable in nonmucoid strains. Mobility shift and Western blot assays revealed that algZ expression requires the sigma factor AlgT. The mapped algZ transcription start site revealed a consensus AlgT-dependent promoter that, when mutated, substantially reduced algZ transcription.

Links

PubMed PMC296253

Keywords

Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; DNA-Binding Proteins/genetics; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Pseudomonas aeruginosa/genetics; Repressor Proteins/genetics; Sigma Factor/genetics; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

PSEAE:RPSH

enables

GO:0000976: transcription regulatory region sequence-specific DNA binding

ECO:0005667: site-directed mutagenesis phenotypic evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:RPSH

enables

GO:0001216: DNA-binding transcription activator activity

ECO:0005667: site-directed mutagenesis phenotypic evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:RPSH

part_of

GO:0032993: protein-DNA complex

ECO:0005667: site-directed mutagenesis phenotypic evidence used in manual assertion

C

Seeded From UniProt

complete

PSEAE:RPSH

involved_in

GO:0032885: regulation of polysaccharide biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:RPSH

enables

GO:0000976: transcription regulatory region sequence-specific DNA binding

ECO:0005667: site-directed mutagenesis evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:RPSH

enables

GO:0001216: DNA-binding transcription activator activity

ECO:0005667: site-directed mutagenesis evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:RPSH

part_of

GO:0032993: protein-DNA complex

ECO:0005667: site-directed mutagenesis evidence used in manual assertion

C

Seeded From UniProt

complete

PSEAE:RPSH

involved_in

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0005657: primer extension assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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