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PMID:14645293
| Citation |
Wozniak, DJ, Sprinkle, AB and Baynham, PJ (2003) Control of Pseudomonas aeruginosa algZ expression by the alternative sigma factor AlgT. J. Bacteriol. 185:7297-300 |
|---|---|
| Abstract |
AlgZ controls Pseudomonas aeruginosa alginate synthesis by activating algD, yet algZ expression is not detectable in nonmucoid strains. Mobility shift and Western blot assays revealed that algZ expression requires the sigma factor AlgT. The mapped algZ transcription start site revealed a consensus AlgT-dependent promoter that, when mutated, substantially reduced algZ transcription. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; DNA-Binding Proteins/genetics; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Pseudomonas aeruginosa/genetics; Repressor Proteins/genetics; Sigma Factor/genetics; Transcription, Genetic |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0000976: transcription regulatory region sequence-specific DNA binding |
ECO:0005667: site-directed mutagenesis phenotypic evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0001216: DNA-binding transcription activator activity |
ECO:0005667: site-directed mutagenesis phenotypic evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0032993: protein-DNA complex |
ECO:0005667: site-directed mutagenesis phenotypic evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0032885: regulation of polysaccharide biosynthetic process |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0000976: transcription regulatory region sequence-specific DNA binding |
ECO:0005667: site-directed mutagenesis evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0001216: DNA-binding transcription activator activity |
ECO:0005667: site-directed mutagenesis evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0032993: protein-DNA complex |
ECO:0005667: site-directed mutagenesis evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045893: positive regulation of transcription, DNA-templated |
ECO:0005657: primer extension assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
Notes
See also
References
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