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PMID:14636573

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Citation

Wallberg, AE, Yamamura, S, Malik, S, Spiegelman, BM and Roeder, RG (2003) Coordination of p300-mediated chromatin remodeling and TRAP/mediator function through coactivator PGC-1alpha. Mol. Cell 12:1137-49

Abstract

Transcriptional coactivators showing physical and functional interactions with PPARgamma include the protein acetyl transferase p300, the TRAP/Mediator complex that interacts with the general transcription machinery, and the highly regulated PGC-1alpha. We show that PGC-1alpha directly interacts with TRAP/Mediator, through the PPARgamma-interacting subunit TRAP220, and stimulates TRAP/Mediator-dependent function on DNA templates. Further, while ineffective by itself, PGC-1alpha stimulates p300-dependent histone acetylation and transcription on chromatin templates in response to PPARgamma. These functions are mediated by largely independent PPARgamma, p300, and TRAP220 interaction domains in PGC-1alpha, whereas p300 and TRAP220 show ligand-dependent interactions with a common region of PPARgamma. Apart from showing PGC-1alpha functions both in chromatin remodeling and in preinitiation complex formation or function (transcription), these results suggest a key role for PGC-1alpha, through concerted but dynamic interactions, in coordinating these steps.

Links

PubMed

Keywords

Animals; Chromatin/metabolism; E1A-Associated p300 Protein; Genes, Reporter; HeLa Cells; Humans; Mediator Complex; Mediator Complex Subunit 1; Mice; Nuclear Proteins/metabolism; Protein Subunits/metabolism; Receptors, Cytoplasmic and Nuclear/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Trans-Activators/metabolism; Transcription Factors/metabolism; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:PRGC1

enables

GO:0003712: transcription coregulator activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

HUMAN:PRGC1

involved_in

GO:0006367: transcription initiation from RNA polymerase II promoter

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PRGC1

involved_in

GO:0065003: protein-containing complex assembly

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PRGC1

involved_in

GO:0035066: positive regulation of histone acetylation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PRGC1

involved_in

GO:0050821: protein stabilization

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete


See also

References

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