PMID:14617139

Citation	Wadhams, GH, Warren, AV, Martin, AC and Armitage, JP (2003) Targeting of two signal transduction pathways to different regions of the bacterial cell. Mol. Microbiol. 50:763-70
Abstract	Components of bacterial chemosensory pathways which sense via transmembrane receptors have been shown to localize to the cell poles. Many species, however, have operons encoding multiple putative chemosensory pathways, some including putative cytoplasmic receptors. In-genome fusions to single or multiple genes encoding components of two chemosensory pathways in Rhodobacter sphaeroides, cheOp2 and cheOp3, revealed that while sensory transducing proteins associated with transmembrane receptors and encoded on cheOp2 were targeted to the cell poles, the proteins associated with putative cytoplasmic receptors and encoded on cheOp3 were all targeted to a cytoplasmic cluster. No proteins were localized to both sites. These data show that bacteria target components of related pathways to different sites in the cell, presumably preventing direct cross-talk between the different pathways, but allowing a balanced response between extracellular and cytoplasmic signals. It also indicates that there is intracellular organization in bacterial cells, with specific proteins targeted and localized to cytoplasmic regions.
Links	PubMed
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Membrane/metabolism; Chemotaxis/physiology; Cytoplasm/genetics; Cytoplasm/metabolism; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Methyltransferases/genetics; Methyltransferases/metabolism; Microscopy, Electron/methods; Operon; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Rhodobacter sphaeroides/genetics; Rhodobacter sphaeroides/metabolism; Signal Transduction
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RHOCB:D5AU71		GO:0006935 : chemotaxis	ECO:0000314:		P		localized exclusively to the cell poles.	complete
RHOSH:CHEB3		GO:0006935: chemotaxis	ECO:0000314:		P		Fluorescently tagged proteins from cheOp2	complete

References

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