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PMID:14608372
| Citation |
Neumann, S, Petfalski, E, Brügger, B, Grosshans, H, Wieland, F, Tollervey, D and Hurt, E (2003) Formation and nuclear export of tRNA, rRNA and mRNA is regulated by the ubiquitin ligase Rsp5p. EMBO Rep. 4:1156-62 |
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| Abstract |
The yeast ubiquitin-protein ligase Rsp5p regulates processes as diverse as polII transcription and endocytosis. Here, we identify Rsp5p in a screen for tRNA export (tex) mutants. The tex23-1/rsp5-3 mutant, which is complemented by RSP5, not only shows a strong nuclear accumulation of tRNAs at the restrictive temperature, but also is severely impaired in the nuclear export of mRNAs and 60S pre-ribosomal subunits. In contrast, nuclear localization sequence (NLS)-mediated nuclear protein import is unaffected in this mutant. Strikingly, the nuclear RNA export defects seen in the rsp5-3 strain are accompanied by a dramatic inhibition of both rRNA and tRNA processing, a combination of phenotypes that has not been reported for any previously characterized mutation in yeast. These data implicate ubiquitination as a mechanism coordinating the major nuclear RNA biogenesis pathways. |
| Links |
PubMed PMC1326418 Online version:10.1038/sj.embor.7400026 |
| Keywords |
Endosomal Sorting Complexes Required for Transport; Exocytosis; Hybridization, Genetic; Lipids/analysis; Lipids/biosynthesis; Microscopy, Electron; Mutation; Phenotype; Plasmids; RNA Polymerase II/genetics; RNA Polymerase II/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA, Ribosomal/genetics; RNA, Ribosomal/metabolism; RNA, Transfer/genetics; RNA, Transfer/metabolism; Ribosomes/chemistry; Ribosomes/metabolism; Saccharomyces cerevisiae Proteins/analysis; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Transcription Factors; Ubiquitin-Protein Ligase Complexes/analysis; Ubiquitin-Protein Ligase Complexes/genetics; Ubiquitin-Protein Ligase Complexes/metabolism; Yeasts/genetics; Yeasts/metabolism; Yeasts/ultrastructure |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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