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PMID:14607906

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Citation

Kawamura, T, Koka, R, Ma, A and Kumar, V (2003) Differential roles for IL-15R alpha-chain in NK cell development and Ly-49 induction. J. Immunol. 171:5085-90

Abstract

IL-15Ralpha-deficient (IL-15Ralpha(-/-)) mice lack NK cells. However, when bone marrow (BM) progenitors from IL-15Ralpha(-/-) mice were cultured with IL-7, stem cell factor and flt3 ligand, followed by IL-15, they were able to differentiate into functional NK cells, indicating that IL-15Ralpha is not critical for NK cell development. Whereas NK cells generated in vitro from IL-15Ralpha(-/-) BM progenitors expressed CD94/NKG2, they failed to express Ly-49 receptors. In keeping with this, when IL-15Ralpha(-/-) BM cells were transferred into wild type recipients, they gave rise to NK cells in vivo, but with greatly reduced expression of Ly-49 receptors. Furthermore, the small numbers of NK cells found in IL-15(-/-) as well as IL-15Ralpha(-/-) but not flt3 ligand(-/-) mice expressed much lower levels of Ly-49 receptors than those from wild type mice. These results indicate a novel role for IL-15Ralpha-chain in Ly-49 induction on developing NK cells.

Links

PubMed

Keywords

Animals; Antigens, Ly/biosynthesis; Antigens, Ly/metabolism; Bone Marrow Cells/cytology; Bone Marrow Cells/immunology; Bone Marrow Cells/metabolism; Bone Marrow Transplantation/immunology; Cell Differentiation/genetics; Cell Differentiation/immunology; Cells, Cultured; Down-Regulation/genetics; Down-Regulation/immunology; Hematopoietic Stem Cells/cytology; Hematopoietic Stem Cells/immunology; Hematopoietic Stem Cells/metabolism; Interleukin-15/deficiency; Interleukin-15/genetics; Interleukin-15/metabolism; Killer Cells, Natural/cytology; Killer Cells, Natural/immunology; Killer Cells, Natural/metabolism; Lectins, C-Type; Mice; Mice, Inbred C57BL; Mice, Knockout; Protein Subunits/biosynthesis; Protein Subunits/deficiency; Protein Subunits/genetics; Protein Subunits/physiology; Radiation Chimera/genetics; Radiation Chimera/immunology; Receptors, Immunologic/antagonists & inhibitors; Receptors, Immunologic/biosynthesis; Receptors, Interleukin-15; Receptors, Interleukin-2/biosynthesis; Receptors, Interleukin-2/deficiency; Receptors, Interleukin-2/genetics; Receptors, Interleukin-2/physiology; Receptors, NK Cell Lectin-Like

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:KLRA1

located_in

GO:0009897: external side of plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:KLRBC

located_in

GO:0009897: external side of plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:IL15

enables

GO:0005125: cytokine activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:IL15

involved_in

GO:0032819: positive regulation of natural killer cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:IL15

involved_in

GO:0032825: positive regulation of natural killer cell differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:IL15

involved_in

GO:0001779: natural killer cell differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:IL15

involved_in

GO:0001787: natural killer cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:IL15

involved_in

GO:0032825: positive regulation of natural killer cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2136897

P

Seeded From UniProt

complete

MOUSE:IL15

involved_in

GO:0001779: natural killer cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2136897

P

Seeded From UniProt

complete

MOUSE:FLT3L

involved_in

GO:0032825: positive regulation of natural killer cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158499

P

Seeded From UniProt

complete

MOUSE:FLT3L

involved_in

GO:0001779: natural killer cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158499

P

Seeded From UniProt

complete

MOUSE:KLRA4

located_in

GO:0009897: external side of plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:KLRA7

located_in

GO:0009897: external side of plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:I15RA

involved_in

GO:0032825: positive regulation of natural killer cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1934016

P

Seeded From UniProt

complete

MOUSE:I15RA

involved_in

GO:0001779: natural killer cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1934016

P

Seeded From UniProt

complete


See also

References

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