PMID:14607906

Citation	Kawamura, T, Koka, R, Ma, A and Kumar, V (2003) Differential roles for IL-15R alpha-chain in NK cell development and Ly-49 induction. J. Immunol. 171:5085-90
Abstract	IL-15Ralpha-deficient (IL-15Ralpha(-/-)) mice lack NK cells. However, when bone marrow (BM) progenitors from IL-15Ralpha(-/-) mice were cultured with IL-7, stem cell factor and flt3 ligand, followed by IL-15, they were able to differentiate into functional NK cells, indicating that IL-15Ralpha is not critical for NK cell development. Whereas NK cells generated in vitro from IL-15Ralpha(-/-) BM progenitors expressed CD94/NKG2, they failed to express Ly-49 receptors. In keeping with this, when IL-15Ralpha(-/-) BM cells were transferred into wild type recipients, they gave rise to NK cells in vivo, but with greatly reduced expression of Ly-49 receptors. Furthermore, the small numbers of NK cells found in IL-15(-/-) as well as IL-15Ralpha(-/-) but not flt3 ligand(-/-) mice expressed much lower levels of Ly-49 receptors than those from wild type mice. These results indicate a novel role for IL-15Ralpha-chain in Ly-49 induction on developing NK cells.
Links	PubMed
Keywords	Animals; Antigens, Ly/biosynthesis; Antigens, Ly/metabolism; Bone Marrow Cells/cytology; Bone Marrow Cells/immunology; Bone Marrow Cells/metabolism; Bone Marrow Transplantation/immunology; Cell Differentiation/genetics; Cell Differentiation/immunology; Cells, Cultured; Down-Regulation/genetics; Down-Regulation/immunology; Hematopoietic Stem Cells/cytology; Hematopoietic Stem Cells/immunology; Hematopoietic Stem Cells/metabolism; Interleukin-15/deficiency; Interleukin-15/genetics; Interleukin-15/metabolism; Killer Cells, Natural/cytology; Killer Cells, Natural/immunology; Killer Cells, Natural/metabolism; Lectins, C-Type; Mice; Mice, Inbred C57BL; Mice, Knockout; Protein Subunits/biosynthesis; Protein Subunits/deficiency; Protein Subunits/genetics; Protein Subunits/physiology; Radiation Chimera/genetics; Radiation Chimera/immunology; Receptors, Immunologic/antagonists & inhibitors; Receptors, Immunologic/biosynthesis; Receptors, Interleukin-15; Receptors, Interleukin-2/biosynthesis; Receptors, Interleukin-2/deficiency; Receptors, Interleukin-2/genetics; Receptors, Interleukin-2/physiology; Receptors, NK Cell Lectin-Like
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:KLRA1	located_in	GO:0009897: external side of plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:KLRBC	located_in	GO:0009897: external side of plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:IL15	enables	GO:0005125: cytokine activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
MOUSE:IL15	involved_in	GO:0032819: positive regulation of natural killer cell proliferation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:IL15	involved_in	GO:0032825: positive regulation of natural killer cell differentiation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:IL15	involved_in	GO:0001779: natural killer cell differentiation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:IL15	involved_in	GO:0001787: natural killer cell proliferation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:IL15	involved_in	GO:0032825: positive regulation of natural killer cell differentiation	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2136897	P		Seeded From UniProt	complete
MOUSE:IL15	involved_in	GO:0001779: natural killer cell differentiation	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2136897	P		Seeded From UniProt	complete
MOUSE:FLT3L	involved_in	GO:0032825: positive regulation of natural killer cell differentiation	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158499	P		Seeded From UniProt	complete
MOUSE:FLT3L	involved_in	GO:0001779: natural killer cell differentiation	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158499	P		Seeded From UniProt	complete
MOUSE:KLRA4	located_in	GO:0009897: external side of plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:KLRA7	located_in	GO:0009897: external side of plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:I15RA	involved_in	GO:0032825: positive regulation of natural killer cell differentiation	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:1934016	P		Seeded From UniProt	complete
MOUSE:I15RA	involved_in	GO:0001779: natural killer cell differentiation	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:1934016	P		Seeded From UniProt	complete

References

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