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PMID:14597206

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Citation

Hapiak, V, Hresko, MC, Schriefer, LA, Saiyasisongkhram, K, Bercher, M and Plenefisch, J (2003) mua-6, a gene required for tissue integrity in Caenorhabditis elegans, encodes a cytoplasmic intermediate filament. Dev. Biol. 263:330-42

Abstract

Locomotion in Caenorhabditis elegans requires force transmission through a network of proteins linking the skeletal muscle, via an intervening basal lamina and epidermis (hypodermis), to the cuticle. Mutations in mua-6 result in hypodermal rupture, muscle detachment from the bodywall, and progressive paralysis. It is shown that mua-6 encodes the cytoplasmic intermediate filament (cIF) A2 protein and that a MUA-6/IFA-2::GFP fusion protein that rescues the presumptive mua-6 null allele localizes to hypodermal hemidesmosomes. This result is consistent with what is known about the function of cIFs in vertebrates. Although MUA-6/IFA-2 is expressed embryonically, and plays an essential postembryonic role in tissue integrity, it is not required for embryonic development of muscle-cuticle linkages nor for the localization of other cIFs or hemidesmosome-associated proteins in the embryo. Finally, the molecular lesion in the mua-6(rh85) allele suggests that the head domain of the MUA-6/IFA-2 is dispensable for its function.

Links

PubMed

Keywords

Animals; Caenorhabditis elegans/embryology; Caenorhabditis elegans/genetics; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/physiology; Genes, Helminth/physiology; Hemidesmosomes/chemistry; Intermediate Filament Proteins/genetics; Intermediate Filament Proteins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:IFA2

located_in

GO:0030056: hemidesmosome

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(WBbt:0005331)

Seeded From UniProt

complete

CAEEL:IFA2

involved_in

GO:0098609: cell-cell adhesion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:IFA2

part_of

GO:0030056: hemidesmosome

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(WBbt:0005331)

Seeded From UniProt

complete


See also

References

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