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PMID:14595002

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Citation

Cabana, VG, Feng, N, Reardon, CA, Lukens, J, Webb, NR, de Beer, FC and Getz, GS (2004) Influence of apoA-I and apoE on the formation of serum amyloid A-containing lipoproteins in vivo and in vitro. J. Lipid Res. 45:317-25

Abstract

Serum amyloid A (SAA) circulates bound to HDL3 during the acute-phase response (APR), and recent evidence suggests that elevated levels of SAA may be a risk factor for cardiovascular disease. In this study, SAA-HDL was produced in vivo during the APR and without the APR by injection of an adenoviral vector expressing human SAA-1. SAA-HDL was also produced in vitro by incubating mouse HDL with recombinant mouse SAA and by SAA-expressing cultured hepatoma cells. Whether produced in vivo or in vitro, SAA-HDL floated at a density corresponding to that of human HDL3 (d 1.12 g/ml) separate from other apolipoproteins, including apolipoprotein A-I (apoA-I; d 1.10 g/ml) when either apoA-I or apolipoprotein E (apoE) was present. In the absence of both apoA-I and apoE, SAA was found in VLDL and LDL, with low levels in the HDL and the lipid-poor fractions suggesting that other HDL apolipoproteins are incapable of facilitating the formation of SAA-HDL. We conclude that SAA does not exist in plasma as a lipid-free protein. In the presence of HDL-associated apoA-I or apoE, SAA circulates as SAA-HDL with a density corresponding to that of human HDL3. In the absence of both apoA-I and apoE, SAA-HDL is not formed and SAA associates with any available lipoprotein.

Links

PubMed Online version:10.1194/jlr.M300414-JLR200

Keywords

Acute-Phase Reaction/blood; Adenoviridae/genetics; Animals; Apolipoprotein A-I/genetics; Apolipoprotein A-I/metabolism; Apolipoproteins E/metabolism; Cardiovascular Diseases/etiology; Cardiovascular Diseases/metabolism; Cell Line, Tumor; DNA-Binding Proteins/genetics; Lipoproteins, HDL/blood; Lipoproteins, HDL3; Mice; Mice, Knockout; Protein Binding; Serum Amyloid A Protein/metabolism; Transduction, Genetic

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

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