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PMID:14592989

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Citation

Stüven, T, Hartmann, E and Görlich, D (2003) Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes. EMBO J. 22:5928-40

Abstract

Active macromolecular transport between the nucleus and cytoplasm proceeds through nuclear pore complexes and is mostly mediated by transport receptors of the importin beta-superfamily. Here we identify exportin 6 (Exp6) as a novel family member from higher eukaryotes and show that it mediates nuclear export of profilin.actin complexes. Exp6 appears to contact primarily actin, but the interaction is greatly enhanced by the presence of profilin. Profilin thus functions not only as the nucleotide exchange factor for actin, but can also be regarded as a cofactor of actin export and hence as a suppressor of actin polymerization in the nucleus. Even though human and Drosophila Exp6 share only approximately 20% identical amino acid residues, their function in profilin.actin export is conserved. A knock-down of Drosophila Exp6 by RNA interference abolishes nuclear exclusion of actin and results in the appearance of nuclear actin paracrystals. In contrast to a previous report, we found no indications of a major and direct role for CRM1 in actin export from mammalian or insect nuclei.

Links

PubMed PMC275422 Online version:10.1093/emboj/cdg565

Keywords

Actins/metabolism; Active Transport, Cell Nucleus; Amino Acid Sequence; Animals; Cloning, Molecular; Contractile Proteins; Drosophila; Drosophila Proteins; HeLa Cells; Humans; Karyopherins/genetics; Karyopherins/metabolism; Microfilament Proteins/metabolism; Molecular Sequence Data; Profilins; Protein Binding; Protein Isoforms/genetics; Protein Isoforms/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Homology, Amino Acid; Solubility; Vertebrates

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:ACTB

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q96QU8

F

Seeded From UniProt

complete

HUMAN:XPO6

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P60709

F

Seeded From UniProt

complete

HUMAN:XPO6

located_in

GO:0005634: nucleus

ECO:0000305: curator inference used in manual assertion

GO:0006611

C

Seeded From UniProt

complete

HUMAN:XPO6

located_in

GO:0005737: cytoplasm

ECO:0000305: curator inference used in manual assertion

GO:0006611

C

Seeded From UniProt

complete

HUMAN:XPO6

involved_in

GO:0006611: protein export from nucleus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:XPO6

enables

GO:0005049: nuclear export signal receptor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

See also

References

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