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PMID:14511394
| Citation |
Hawley, SA, Boudeau, J, Reid, JL, Mustard, KJ, Udd, L, Mäkelä, TP, Alessi, DR and Hardie, DG (2003) Complexes between the LKB1 tumor suppressor, STRAD alpha/beta and MO25 alpha/beta are upstream kinases in the AMP-activated protein kinase cascade. J. Biol. 2:28 |
|---|---|
| Abstract |
The AMP-activated protein kinase (AMPK) cascade is a sensor of cellular energy charge that acts as a 'metabolic master switch' and inhibits cell proliferation. Activation requires phosphorylation of Thr172 of AMPK within the activation loop by upstream kinases (AMPKKs) that have not been identified. Recently, we identified three related protein kinases acting upstream of the yeast homolog of AMPK. Although they do not have obvious mammalian homologs, they are related to LKB1, a tumor suppressor that is mutated in the human Peutz-Jeghers cancer syndrome. We recently showed that LKB1 exists as a complex with two accessory subunits, STRAD alpha/beta and MO25 alpha/beta. |
| Links |
PubMed PMC333410 Online version:10.1186/1475-4924-2-28 |
| Keywords |
AMP-Activated Protein Kinases; Adaptor Proteins, Signal Transducing/metabolism; Adaptor Proteins, Vesicular Transport/metabolism; Animals; Catalytic Domain; Cell Line; Cell Line, Tumor; Cell-Free System; Embryo, Mammalian/cytology; Enzyme Activation/physiology; Fibroblasts/enzymology; Fibroblasts/metabolism; Genes, Tumor Suppressor; HeLa Cells/chemistry; HeLa Cells/enzymology; HeLa Cells/metabolism; HeLa Cells/pathology; Humans; Immunoprecipitation/methods; Kidney/chemistry; Kidney/cytology; Kidney/embryology; Kidney/enzymology; Liver/enzymology; Mice; Multienzyme Complexes/metabolism; Multienzyme Complexes/physiology; Multiprotein Complexes/metabolism; Multiprotein Complexes/physiology; Protein Kinases/chemistry; Protein Kinases/metabolism; Protein Subunits/metabolism; Protein-Serine-Threonine Kinases/biosynthesis; Protein-Serine-Threonine Kinases/chemistry; Protein-Serine-Threonine Kinases/deficiency; Protein-Serine-Threonine Kinases/metabolism; Protein-Serine-Threonine Kinases/physiology; Rats; Recombinant Proteins |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
RAT:A0A0G2JZH0 |
part_of |
GO:0032991: protein-containing complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
RAT:D3ZJ77 |
part_of |
GO:0032991: protein-containing complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
RAT:STK11 |
enables |
GO:0044877: protein-containing complex binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
RAT:STK11 |
part_of |
GO:0032991: protein-containing complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
RAT:STK11 |
enables |
GO:0044877: protein-containing complex binding |
ECO:0000353: physical interaction evidence used in manual assertion |
RGD:1306390 |
F |
Seeded From UniProt |
complete | |
|
RAT:STK11 |
enables |
GO:0004674: protein serine/threonine kinase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0044877: protein-containing complex binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
RAT:AAKG1 |
enables |
GO:0044877: protein-containing complex binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
RAT:AAKG1 |
contributes_to |
GO:0004679: AMP-activated protein kinase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
RAT:AAKG1 |
part_of |
GO:0032991: protein-containing complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
RAT:AAKB1 |
part_of |
GO:0032991: protein-containing complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
RAT:AAPK2 |
enables |
GO:0044877: protein-containing complex binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
RAT:AAPK2 |
enables |
GO:0004679: AMP-activated protein kinase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
RAT:AAPK2 |
part_of |
GO:0032991: protein-containing complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
RAT:STRAA |
enables |
GO:0044877: protein-containing complex binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
RAT:STRAA |
part_of |
GO:0032991: protein-containing complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
RAT:STRAA |
contributes_to |
GO:0004674: protein serine/threonine kinase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
RAT:STRAA |
involved_in |
GO:0032147: activation of protein kinase activity |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0051291: protein heterooligomerization |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0032991: protein-containing complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004679: AMP-activated protein kinase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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