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PMID:14500984

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Citation

Chen, JG, Willard, FS, Huang, J, Liang, J, Chasse, SA, Jones, AM and Siderovski, DP (2003) A seven-transmembrane RGS protein that modulates plant cell proliferation. Science 301:1728-31

Abstract

G protein-coupled receptors (GPCRs) at the cell surface activate heterotrimeric G proteins by inducing the G protein alpha (Galpha) subunit to exchange guanosine diphosphate for guanosine triphosphate. Regulators of G protein signaling (RGS) proteins accelerate the deactivation of Galpha subunits to reduce GPCR signaling. Here we identified an RGS protein (AtRGS1) in Arabidopsis that has a predicted structure similar to a GPCR as well as an RGS box with GTPase accelerating activity. Expression of AtRGS1 complemented the pheromone supersensitivity phenotype of a yeast RGS mutant, sst2Delta. Loss of AtRGS1 increased the activity of the Arabidopsis Galpha subunit, resulting in increased cell elongation in hypocotyls in darkness and increased cell production in roots grown in light. These findings suggest that AtRGS1 is a critical modulator of plant cell proliferation.

Links

PubMed Online version:10.1126/science.1087790

Keywords

Alleles; Amino Acid Sequence; Arabidopsis/cytology; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cell Differentiation; Cell Division; Cell Membrane/metabolism; GTP-Binding Protein alpha Subunits; Heterotrimeric GTP-Binding Proteins/metabolism; Meristem/metabolism; Mitosis; Molecular Sequence Data; Mutation; Open Reading Frames; Phenotype; Plant Roots/cytology; Plant Roots/growth & development; Plant Roots/metabolism; Protein Precursors/metabolism; Protein Structure, Tertiary; RGS Proteins/chemistry; RGS Proteins/genetics; RGS Proteins/metabolism; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/metabolism; Transgenes

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:GPA1

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:GPA1

enables

GO:0003924: GTPase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:GPA1

acts_upstream_of_or_within

GO:0042127: regulation of cell population proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RGS1

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:RGS1

enables

GO:0005096: GTPase activator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:RGS1

acts_upstream_of_or_within

GO:0043547: positive regulation of GTPase activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RGS1

acts_upstream_of_or_within

GO:0042127: regulation of cell population proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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