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This is your chance to make annotations OR challenge other team's annotations. You may also DEFEND or suggest improvements to your own annotations IF they have been challenged. Please note, although we ENCOURAGE challenges, an excess of identical challenges that do not appear to be applicable to the annotation or well thought out will be considered spam and ignored.

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PMID:1421759

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Citation

Kumar, R, Yang, J, Eddy, RL, Byers, MG, Shows, TB and Stanley, P (1992) Cloning and expression of the murine gene and chromosomal location of the human gene encoding N-acetylglucosaminyltransferase I. Glycobiology 2:383-93

Abstract

A mouse cDNA clone previously isolated from an F9 teratocarcinoma cell library and shown to confer N-acetylglucosaminyltransferase I (GlcNAc-TI) activity on Lec1 Chinese hamster ovary (CHO) cell transfectants [Kumar, R., Yang,J., Larsen,R.D. and Stanley,P. (1990) Proc. Natl. Acad Sci. USA, 87, 9948-9952] has been sequenced. The nucleotide and deduced amino acid sequences are highly homologous to previously described human and rabbit GlcNAc-TI cDNAs. A 1250 bp portion of the mouse cDNA encoding all but the first 34 amino acids of the deduced protein sequence was inducibly expressed in Escherichia coli and gave rise to a prominent fusion protein of mol. wt approximately 45 kDa whose presence correlated with high levels of GlcNAc-TI activity in cell lysates. Probes generated from the cDNA were used to show that the GlcNAc-TI gene is present in a single copy in mammals and that a homologous gene was not detectable (under low-stringency hybridization conditions) in DNA from yeast, sea urchin, Drosophila or Chaenorhaditis elegans. Genomic DNA clones that hybridized to probes generated from the GlcNAc-TI cDNA were isolated from a mouse liver library. Restriction analyses, Southern hybridization and DNA sequence analyses of subcloned genomic DNA fragments and a polymerase chain reaction (PCR) product provided evidence that the coding and 3' untranslated regions of the cDNA reside in a single exon. However, the mouse GlcNAc-TI gene (Mgat-1) includes at least one additional exon 5' of the coding region. Southern analyses of DNA from mouse-human somatic cell hybrids and in situ hybridization were used to locate the human GlcNAc-TI gene (MGAT-1) between positions q31.2 and q31.3 on chromosome 5, a region of chromosome 5 that is syntenic with a region of mouse chromosome 11. Northern analyses of adult mouse tissues revealed two GlcNAc-TI gene transcripts that are differentially expressed in different tissues.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Bacteriophage lambda/genetics; Base Sequence; CHO Cells/chemistry; Cattle; Chromosome Mapping; Chromosomes, Human, Pair 5; Cloning, Molecular; Cricetinae; DNA/chemistry; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression; Humans; Mice; Molecular Sequence Data; N-Acetylglucosaminyltransferases/genetics; Nucleic Acid Hybridization; Sequence Homology; Species Specificity; Transformation, Bacterial

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

MGI:Mgat1

GO:0008375

acetylglucosaminyltransferase activity

IDA: Inferred from Direct Assay

F

From MGI


MGI:Mgat1

GO:0006049

UDP-N-acetylglucosamine catabolic process

IDA: Inferred from Direct Assay

P

From MGI



See also

References

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