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PMID:1331806

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Citation

Phillips, GJ and Silhavy, TJ (1992) The E. coli ffh gene is necessary for viability and efficient protein export. Nature 359:744-6

Abstract

Homologues of the gene encoding the 54K (M(r) 54,000) subunit of the mammalian signal recognition particle have been identified in different organisms. The Escherichia coli homologue, termed ffh (for fifty-four homologue), specifies a protein (Ffh) that shares many properties with its eukaryotic counterpart, including association with mammalian 7S RNA and the ability to bind signal sequences specifically. Ffh also associates with E. coli 4.5S RNA, showing that it can form a ribonucleoprotein complex in prokaryotes. These results are intriguing because extensive genetic and biochemical characterization of E. coli failed to identify a signal recognition particle-like mechanism for protein export. Here we address this issue directly by construction of a strain in which ffh expression is arabinose-dependent. Results of depletion experiments indicate that Ffh is important in protein translocation.

Links

PubMed Online version:10.1038/359744a0

Keywords

Bacterial Proteins/metabolism; Biological Transport; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/metabolism; Exocytosis; Genes, Bacterial; Proteins/secretion; Ribonucleoproteins/chemistry; Ribonucleoproteins/genetics; Signal Recognition Particle

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:SRP54

acts_upstream_of_or_within

GO:0006612: protein targeting to membrane

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:SRP54

involved_in

GO:0006612: protein targeting to membrane

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:SRP54

part_of

GO:0048500: signal recognition particle

ECO:0000315: mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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