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PMID:1326540

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Citation

Cheifetz, S, Bellón, T, Calés, C, Vera, S, Bernabeu, C, Massagué, J and Letarte, M (1992) Endoglin is a component of the transforming growth factor-beta receptor system in human endothelial cells. J. Biol. Chem. 267:19027-30

Abstract

Endoglin, a dimeric membrane glycoprotein expressed at high levels on human vascular endothelial cells, shares regions of sequence identity with betaglycan, a major binding protein for transforming growth factor-beta (TGF-beta) that co-exists with TGF-beta receptors I and II in a variety of cell lines but is low or absent in endothelial cells. We have examined whether endoglin also binds TGF-beta and demonstrate here that the major TGF-beta 1-binding protein co-existing with TGF-beta receptors I and II on human umbilical vein endothelial cells is endoglin, as determined by specific immunoprecipitation of endoglin affinity-labeled with 125I-TGF-beta. Furthermore, endoglin ectopically expressed in COS cells binds TGF-beta 1. Competition affinity-labeling experiments showed that endoglin binds TGF-beta 1 (KD approximately 50 pM) and TGF-beta 3 with high affinity but fails to bind TGF-beta 2. This difference in affinity of endoglin for the TGF-beta isoforms is in contrast to beta-glycan which recognizes all three isoforms. TGF-beta however is binding with high affinity to only a small fraction of the available endoglin molecules, suggesting that some rate-limiting event is required to sustain TGF-beta binding to endoglin.

Links

PubMed

Keywords

Antigens, CD; Cells, Cultured; Endothelium, Vascular/metabolism; Humans; Macromolecular Substances; Membrane Glycoproteins/immunology; Membrane Glycoproteins/metabolism; Molecular Weight; Precipitin Tests; Receptors, Cell Surface/chemistry; Receptors, Cell Surface/immunology; Receptors, Cell Surface/metabolism; Receptors, Transforming Growth Factor beta; Transfection; Transforming Growth Factor beta/metabolism; Vascular Cell Adhesion Molecule-1

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:TGFB1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P37173

F

Seeded From UniProt

complete

HUMAN:TGFB1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P36897

F

Seeded From UniProt

complete

HUMAN:TGFB1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P17813

F

Seeded From UniProt

complete

HUMAN:TGFB3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P17813

F

Seeded From UniProt

complete

HUMAN:EGLN

enables

GO:0050431: transforming growth factor beta binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P10600

F

Seeded From UniProt

complete

HUMAN:EGLN

enables

GO:0050431: transforming growth factor beta binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P01137

F

Seeded From UniProt

complete

HUMAN:TGFR1

involved_in

GO:0007179: transforming growth factor beta receptor signaling pathway

ECO:0000305: curator inference used in manual assertion

GO:0050431

P

Seeded From UniProt

complete

HUMAN:TGFR1

enables

GO:0005024: transforming growth factor beta-activated receptor activity

ECO:0000305: curator inference used in manual assertion

GO:0050431

F

Seeded From UniProt

complete

HUMAN:TGFR1

enables

GO:0050431: transforming growth factor beta binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P01137

F

Seeded From UniProt

complete

HUMAN:TGFR2

enables

GO:0050431: transforming growth factor beta binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P01137

F

Seeded From UniProt

complete

HUMAN:TGFR2

involved_in

GO:0007179: transforming growth factor beta receptor signaling pathway

ECO:0000305: curator inference used in manual assertion

GO:0050431

P

Seeded From UniProt

complete

HUMAN:TGFR2

enables

GO:0005024: transforming growth factor beta-activated receptor activity

ECO:0000305: curator inference used in manual assertion

GO:0050431

F

Seeded From UniProt

complete


See also

References

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