GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:1313436

From GONUTS
Jump to: navigation, search
Citation

Levin, JZ and Horvitz, HR (1992) The Caenorhabditis elegans unc-93 gene encodes a putative transmembrane protein that regulates muscle contraction. J. Cell Biol. 117:143-55

Abstract

unc-93 is one of a set of five interacting genes involved in the regulation or coordination of muscle contraction in Caenorhabditis elegans. Rare altered-function alleles of unc-93 result in sluggish movement and a characteristic "rubber band" uncoordinated phenotype. By contrast, null alleles cause no visibly abnormal phenotype, presumably as a consequence of the functional redundancy of unc-93. To understand better the role of unc-93 in regulating muscle contraction, we have cloned and molecularly characterized this gene. We isolated transposon-insertion alleles and used them to identify the region of DNA encoding the unc-93 protein. Two unc-93 proteins differing at their NH2 termini are potentially encoded by transcripts that differ at their 5' ends. The putative unc-93 proteins are 700 and 705 amino acids in length and have two distinct regions: the NH2 terminal portion of 240 or 245 amino acids is extremely hydrophilic, whereas the rest of the protein has multiple potential membrane-spanning domains. The unc-93 transcripts are low in abundance and the unc-93 gene displays weak codon usage bias, suggesting that the unc-93 protein is relatively rare. The unc-93 protein has no sequence similarity to proteins listed in current data-bases. Thus, unc-93 is likely to encode a novel membrane-associated muscle protein. We discuss possible roles for the unc-93 protein either as a component of an ion transport system involved in excitation-contraction coupling in muscle or in coordinating muscle contraction between muscle cells by affecting the functioning of gap junctions.

Links

PubMed PMC2289394

Keywords

Alleles; Amino Acid Sequence; Animals; Base Sequence; Caenorhabditis/genetics; Caenorhabditis/physiology; Caenorhabditis elegans Proteins; Cloning, Molecular; DNA/genetics; DNA/isolation & purification; DNA/radiation effects; DNA Transposable Elements; Exons; Gamma Rays; Gene Library; Gene Rearrangement; Helminth Proteins/genetics; Introns; Membrane Proteins/genetics; Molecular Sequence Data; Muscle Contraction; Muscle Proteins/genetics; Muscle Proteins/physiology; Mutagenesis, Insertional; Polymorphism, Genetic; Protein Conformation; Repetitive Sequences, Nucleic Acid; Restriction Mapping; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

See Help:References for how to manage references in GONUTS.