GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:12970194
| Citation |
Roy, A, Solodovnikova, N, Nicholson, T, Antholine, W and Walden, WE (2003) A novel eukaryotic factor for cytosolic Fe-S cluster assembly. EMBO J. 22:4826-35 |
|---|---|
| Abstract |
Iron regulatory protein 1 (IRP1) is regulated through the assembly/disassembly of a [4Fe-4S] cluster, which interconverts IRP1 with cytosolic aconitase. A genetic screen to isolate Saccharomyces cerevisiae strains bearing mutations in genes required for the conversion of IRP1 to c-aconitase led to the identification of a previously uncharacterized, essential gene, which we call CFD1 (cytosolic Fe-S cluster deficient). CFD1 encodes a highly conserved, putative P-loop ATPase. A non-lethal mutation of CFD1 (cfd1-1) reduced c-aconitase specific activity in IRP1-transformed yeast by >90%, although IRP1 in these cells could be readily converted to c-aconitase in vitro upon incubation with iron alone. IRP1-transformed cfd1-1 yeast lacked EPR-detectable Fe-S clusters in c-aconitase, pointing to a defect in Fe-S cluster assembly. The specific activity of another cytosolic Fe-S protein, Leu1p, was also inhibited by >90% in cfd1-1 yeast, whereas activity of mitochondrial Fe-S proteins was not inhibited. Consistent with a cytosolic site of activity, Cfd1p was localized in the cytoplasm. To our knowledge, Cfd1p is the first cytoplasmic Fe-S cluster assembly factor described in eukaryotes. |
| Links |
PubMed PMC212722 Online version:10.1093/emboj/cdg455 |
| Keywords |
Aconitate Hydratase/metabolism; Amino Acid Sequence; Animals; Electron Spin Resonance Spectroscopy; Gene Expression Regulation, Fungal; Genetic Complementation Test; Humans; Iron Regulatory Protein 1/chemistry; Iron Regulatory Protein 1/genetics; Iron Regulatory Protein 1/metabolism; Iron-Sulfur Proteins/chemistry; Iron-Sulfur Proteins/metabolism; Molecular Sequence Data; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Transfection |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
