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PMID:12970156

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Citation

Kaiya, H, Kojima, M, Hosoda, H, Moriyama, S, Takahashi, A, Kawauchi, H and Kangawa, K (2003) Peptide purification, complementary deoxyribonucleic acid (DNA) and genomic DNA cloning, and functional characterization of ghrelin in rainbow trout. Endocrinology 144:5215-26

Abstract

We have identified ghrelin from the stomach of rainbow trout. Four isoforms of ghrelin peptide were isolated: the C-terminal amidated type of rainbow trout ghrelin (rt ghrelin) composed of 24 amino acids (GSSFLSPSQKPQVRQGKGKPPRV-amide) is a basic form; des-VRQ-rt ghrelin, which deleted three amino acids (V13R14Q15) from rt ghrelin; and further two types of rt ghrelin that retained the glycine residue at the C terminus, rt ghrelin-Gly, and des-VRQ-rt ghrelin-Gly. The third serine residue was modified by octanoic acid, decanoic acid, or the unsaturated form of those fatty acids. In agreement with the isolated peptides, two cDNAs of different lengths were isolated. The rt ghrelin gene has five exons and four introns, and two different mRNA molecules are predicted to be produced by alternative splicing of the gene. A high level of ghrelin mRNA expression was detected in the stomach, and moderate levels were detected in the brain, hypothalamus, and intestinal tracts. Des-VRQ-rt ghrelin stimulated the release of GH in the rat in vivo. Furthermore, des-VRQ-rt ghrelin stimulated the release of GH, but not the release of prolactin and somatolactin in rainbow trout in vivo and in vitro. These results indicate that ghrelin is a novel GH secretagogue in rainbow trout that may affect somatic growth or osmoregulation through GH. Because ghrelin is expressed in various tissues other than stomach, it may play important role(s) in cellular function as a local regulator.

Links

PubMed Online version:10.1210/en.2003-1085

Keywords

Amino Acid Sequence; Animals; Base Sequence; CHO Cells; Cloning, Molecular; Cricetinae; DNA, Complementary/genetics; DNA, Complementary/isolation & purification; Fish Proteins; Gene Expression/physiology; Ghrelin; Glycoproteins/metabolism; Growth Hormone/metabolism; Injections, Intraperitoneal; Molecular Sequence Data; Oncorhynchus mykiss/genetics; Peptide Hormones/genetics; Peptide Hormones/isolation & purification; Peptide Hormones/metabolism; Peptide Hormones/pharmacology; Pituitary Gland/drug effects; Pituitary Gland/metabolism; Pituitary Hormones/metabolism; Prolactin/metabolism; RNA, Messenger/metabolism; Stomach/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ONCKE:A8R5N8

involved_in

GO:0030252: growth hormone secretion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ONCKE:A8R5N8

involved_in

GO:0051480: regulation of cytosolic calcium ion concentration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ONCMY:SOMA1

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ONCMY:SOMA2

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CARAU:GHRL

involved_in

GO:0030252: growth hormone secretion

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q76IQ4

P

Seeded From UniProt

complete

ONCMY:GHRL

enables

GO:0001664: G protein-coupled receptor binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q9QYH7

F

Seeded From UniProt

complete

ONCMY:GHRL

involved_in

GO:0007186: G protein-coupled receptor signaling pathway

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q9QYH7

P

Seeded From UniProt

complete

ONCMY:GHRL

enables

GO:0016608: growth hormone-releasing hormone activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q9QYH7

F

Seeded From UniProt

complete

ONCMY:GHRL

involved_in

GO:0030252: growth hormone secretion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ONCMY:GHRL

involved_in

GO:0051480: regulation of cytosolic calcium ion concentration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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