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PMID:12960429

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Citation

Wiederkehr, A, Du, Y, Pypaert, M, Ferro-Novick, S and Novick, P (2003) Sec3p is needed for the spatial regulation of secretion and for the inheritance of the cortical endoplasmic reticulum. Mol. Biol. Cell 14:4770-82

Abstract

Sec3p is a component of the exocyst complex that tethers secretory vesicles to the plasma membrane at exocytic sites in preparation for fusion. Unlike all other exocyst structural genes, SEC3 is not essential for growth. Cells lacking Sec3p grow and secrete surprisingly well at 25 degrees C; however, late markers of secretion, such as the vesicle marker Sec4p and the exocyst subunit Sec8p, localize more diffusely within the bud. Furthermore, sec3Delta cells are strikingly round relative to wild-type cells and are unable to form pointed mating projections in response to alpha factor. These phenotypes support the proposed role of Sec3p as a spatial landmark for secretion. We also find that cells lacking Sec3p exhibit a dramatic defect in the inheritance of cortical ER into the bud, whereas the inheritance of mitochondria and Golgi is unaffected. Overexpression of Sec3p results in a prominent patch of the endoplasmic reticulum (ER) marker Sec61p-GFP at the bud tip. Cortical ER inheritance in yeast has been suggested to involve the capture of ER tubules at the bud tip. Sec3p may act in this process as a spatial landmark for cortical ER inheritance.

Links

PubMed PMC284782 Online version:10.1091/mbc.E03-04-0229

Keywords

Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Membrane/genetics; Cell Membrane/metabolism; Cloning, Molecular; Endoplasmic Reticulum/genetics; Endoplasmic Reticulum/metabolism; Exocytosis/physiology; Microscopy, Electron; Mitochondria; Mutation; Protein Binding; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Vesicular Transport Proteins; rab GTP-Binding Proteins/genetics; rab GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:SEC8

located_in

GO:0005935: cellular bud neck

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:SEC8

located_in

GO:0005934: cellular bud tip

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:SEC3

involved_in

GO:0048309: endoplasmic reticulum inheritance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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