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PMID:12953106

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Citation

Lee, HY, Bahn, SC, Kang, YM, Lee, KH, Kim, HJ, Noh, EK, Palta, JP, Shin, JS and Ryu, SB (2003) Secretory low molecular weight phospholipase A2 plays important roles in cell elongation and shoot gravitropism in Arabidopsis. Plant Cell 15:1990-2002

Abstract

To elucidate the cellular functions of phospholipase A(2) in plants, an Arabidopsis cDNA encoding a secretory low molecular weight phospholipase A(2) (AtsPLA(2)beta) was isolated. Phenotype analyses of transgenic plants showed that overexpression of AtsPLA(2)beta promotes cell elongation, resulting in prolonged leaf petioles and inflorescence stems, whereas RNA interference-mediated silencing of AtsPLA(2)beta expression retards cell elongation, resulting in shortened leaf petioles and stems. AtsPLA(2)beta is expressed in the cortical, vascular, and endodermal cells of the actively growing tissues of inflorescence stems and hypocotyls. AtsPLA(2)beta then is secreted into the extracellular spaces, where signaling for cell wall acidification is thought to occur. AtsPLA(2)beta-overexpressing or -silenced transgenic plants showed altered gravitropism in inflorescence stems and hypocotyls. AtsPLA(2)beta expression is induced rapidly by auxin treatment and in the curving regions of inflorescence stems undergoing the gravitropic response. These results suggest that AtsPLA(2)beta regulates the process of cell elongation and plays important roles in shoot gravitropism by mediating auxin-induced cell elongation.

Links

PubMed PMC181326

Keywords

Amino Acid Sequence; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cell Division/genetics; Cell Division/physiology; Cell Wall/secretion; Cloning, Molecular; DNA, Complementary/chemistry; DNA, Complementary/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Flowers/enzymology; Flowers/genetics; Flowers/growth & development; Gene Expression Regulation, Enzymologic/drug effects; Gene Expression Regulation, Plant/drug effects; Gravitropism/genetics; Gravitropism/physiology; Hypocotyl/enzymology; Hypocotyl/genetics; Hypocotyl/growth & development; Indoleacetic Acids/pharmacology; Molecular Sequence Data; Molecular Weight; Phospholipases A/genetics; Phospholipases A/metabolism; Phospholipases A2; Plant Leaves/enzymology; Plant Leaves/genetics; Plant Leaves/growth & development; Plant Shoots/enzymology; Plant Shoots/genetics; Plant Shoots/growth & development; Plants, Genetically Modified; Protein Sorting Signals/genetics; Protein Sorting Signals/physiology; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Signal Transduction/genetics; Signal Transduction/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:PLA2B

involved_in

GO:0030307: positive regulation of cell growth

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLA2B

involved_in

GO:0009733: response to auxin

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLA2B

involved_in

GO:0009630: gravitropism

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLA2B

enables

GO:0004623: phospholipase A2 activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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