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PMID:12937278

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Citation

Segbert, C, Barkus, R, Powers, J, Strome, S, Saxton, WM and Bossinger, O (2003) KLP-18, a Klp2 kinesin, is required for assembly of acentrosomal meiotic spindles in Caenorhabditis elegans. Mol. Biol. Cell 14:4458-69

Abstract

The proper segregation of chromosomes during meiosis or mitosis requires the assembly of well organized spindles. In many organisms, meiotic spindles lack centrosomes. The formation of such acentrosomal spindles seems to involve first assembly or capture of microtubules (MTs) in a random pattern around the meiotic chromosomes and then parallel bundling and bipolar organization by the action of MT motors and other proteins. Here, we describe the structure, distribution, and function of KLP-18, a Caenorhabditis elegans Klp2 kinesin. Previous reports of Klp2 kinesins agree that it concentrates in spindles, but do not provide a clear view of its function. During prometaphase, metaphase, and anaphase, KLP-18 concentrates toward the poles in both meiotic and mitotic spindles. Depletion of KLP-18 by RNA-mediated interference prevents parallel bundling/bipolar organization of the MTs that accumulate around female meiotic chromosomes. Hence, meiotic chromosome segregation fails, leading to haploid or aneuploid embryos. Subsequent assembly and function of centrosomal mitotic spindles is normal except when aberrant maternal chromatin is present. This suggests that although KLP-18 is critical for organizing chromosome-derived MTs into a parallel bipolar spindle, the order inherent in centrosome-derived astral MT arrays greatly reduces or eliminates the need for KLP-18 organizing activity in mitotic spindles.

Links

PubMed PMC266765 Online version:10.1091/mbc.E03-05-0283

Keywords

Animals; Caenorhabditis elegans/metabolism; Caenorhabditis elegans/physiology; Centrosome/metabolism; Centrosome/physiology; Chromosomes/metabolism; Chromosomes/physiology; Female; Insect Proteins/drug effects; Insect Proteins/metabolism; Insect Proteins/physiology; Kinesin/drug effects; Kinesin/metabolism; Kinesin/physiology; Meiosis/physiology; Microtubules/metabolism; Microtubules/physiology; Mitotic Spindle Apparatus/metabolism; Mitotic Spindle Apparatus/physiology; RNA, Small Interfering/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:G5EBX0

located_in

GO:0005938: cell cortex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:G5EBX0

located_in

GO:0005874: microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:G5EBX0

located_in

GO:0000922: spindle pole

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:G5EBX0

involved_in

GO:0090306: spindle assembly involved in meiosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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