GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:12895416

From GONUTS
Jump to: navigation, search
Citation

Ungless, MA, Singh, V, Crowder, TL, Yaka, R, Ron, D and Bonci, A (2003) Corticotropin-releasing factor requires CRF binding protein to potentiate NMDA receptors via CRF receptor 2 in dopamine neurons. Neuron 39:401-7

Abstract

Stress increases addictive behaviors and is a common cause of relapse. Corticotropin-releasing factor (CRF) plays a key role in the modulation of drug taking by stress. However, the mechanism by which CRF modulates neuronal activity in circuits involved in drug addiction is poorly understood. Here we show that CRF induces a potentiation of NMDAR (N-methyl-D-aspartate receptor)-mediated synaptic transmission in dopamine neurons of the ventral tegmental area (VTA). This effect involves CRF receptor 2 (CRF-R2) and activation of the phospholipase C (PLC)-protein kinase C (PKC) pathway. We also find that this potentiation requires CRF binding protein (CRF-BP). Accordingly, CRF-like peptides, which do not bind the CRF-BP with high affinity, do not potentiate NMDARs. These results provide evidence of the first specific roles for CRF-R2 and CRF-BP in the modulation of neuronal activity and suggest that NMDARs in the VTA may be a target for both drugs of abuse and stress.

Links

PubMed

Keywords

Animals; Carrier Proteins/physiology; Corticotropin-Releasing Hormone/pharmacology; Corticotropin-Releasing Hormone/physiology; Dopamine/metabolism; Male; Mice; Mice, Inbred C57BL; Neurons/drug effects; Neurons/metabolism; Patch-Clamp Techniques; Receptors, Corticotropin-Releasing Hormone/physiology; Receptors, N-Methyl-D-Aspartate/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SHEEP:CRF

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q60571

F

Seeded From UniProt

complete

SHEEP:CRF

involved_in

GO:0001963: synaptic transmission, dopaminergic

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

SHEEP:CRF

involved_in

GO:2000310: regulation of NMDA receptor activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:CRF

involved_in

GO:0001963: synaptic transmission, dopaminergic

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:CRF

involved_in

GO:2000310: regulation of NMDA receptor activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CRF

involved_in

GO:0001963: synaptic transmission, dopaminergic

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CRF

involved_in

GO:2000310: regulation of NMDA receptor activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CRHBP

involved_in

GO:0001963: synaptic transmission, dopaminergic

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CRHBP

involved_in

GO:2000310: regulation of NMDA receptor activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CRHBP

enables

GO:0051424: corticotropin-releasing hormone binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P01142

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.