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PMID:12879074

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Citation

Agathon, A, Thisse, C and Thisse, B (2003) The molecular nature of the zebrafish tail organizer. Nature 424:448-52

Abstract

Based on grafting experiments, Mangold and Spemann showed the dorsal blastopore lip of an amphibian gastrula to be able to induce a secondary body axis. The equivalent of this organizer region has been identified in different vertebrates including teleosts. However, whereas the graft can induce ectopic head and trunk, endogenous and ectopic axes fuse in the posterior part of the body, raising the question of whether a distinct organizer region is necessary for tail development. Here we reveal, by isochronic and heterochronic transplantation, the existence of a tail organizer deriving from the ventral margin of the zebrafish embryo, which is independent of the dorsal Spemann organizer. Loss-of-function experiments reveal that bone morphogenetic protein (BMP), Nodal and Wnt8 signalling pathways are required for tail development. Moreover, stimulation of naive cells by a combination of BMP, Nodal and Wnt8 mimics the tail-organizing activity of the ventral margin and induces surrounding tissues to become tail. In contrast to induction of the vertebrate head, known to result from the triple inhibition of BMP, Nodal and Wnt, here we show that induction of the tail results from the triple stimulation of BMP, Nodal and Wnt8 signalling pathways.

Links

PubMed Online version:10.1038/nature01822

Keywords

Animals; Bone Morphogenetic Proteins/antagonists & inhibitors; Bone Morphogenetic Proteins/metabolism; Bone Morphogenetic Proteins/pharmacology; Cytoskeletal Proteins; Embryonic Induction/drug effects; Nodal Protein; Organizers, Embryonic/physiology; Proteins/antagonists & inhibitors; Proteins/metabolism; Proteins/pharmacology; RNA, Messenger/genetics; RNA, Messenger/metabolism; Signal Transduction/drug effects; Tail/drug effects; Tail/embryology; Tail/metabolism; Transforming Growth Factor beta/metabolism; Transforming Growth Factor beta/pharmacology; Wnt Proteins; Zebrafish/embryology; Zebrafish/genetics; Zebrafish/metabolism; Zebrafish Proteins/antagonists & inhibitors; Zebrafish Proteins/metabolism; Zebrafish Proteins/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:A0A0B5JK91

involved_in

GO:0045165: cell fate commitment

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-980528-2059

P

Seeded From UniProt

complete

DANRE:A0A0R4IZE5

involved_in

GO:0045165: cell fate commitment

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-980528-2059

P

Seeded From UniProt

complete

DANRE:A0A2R8QUR2

involved_in

GO:0045165: cell fate commitment

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-980528-2059

P

Seeded From UniProt

complete

DANRE:F1QAD6

involved_in

GO:0045165: cell fate commitment

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-990415-181

P

Seeded From UniProt

complete

DANRE:F1QAD6

involved_in

GO:0045165: cell fate commitment

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-980526-332

P

Seeded From UniProt

complete

DANRE:O13107

involved_in

GO:0045165: cell fate commitment

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-990415-181

P

Seeded From UniProt

complete

DANRE:O13107

involved_in

GO:0045165: cell fate commitment

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-980526-332

P

Seeded From UniProt

complete

DANRE:P87358

involved_in

GO:0045165: cell fate commitment

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-980528-2059

P

Seeded From UniProt

complete

DANRE:P87380

involved_in

GO:0045165: cell fate commitment

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-990415-181

P

Seeded From UniProt

complete

DANRE:P87380

involved_in

GO:0045165: cell fate commitment

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-980526-332

P

Seeded From UniProt

complete

DANRE:WNT8A

involved_in

GO:0045165: cell fate commitment

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-980528-2059

P

Seeded From UniProt

complete

DANRE:O57574

involved_in

GO:0045165: cell fate commitment

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-990415-181

P

Seeded From UniProt

complete

DANRE:O57574

involved_in

GO:0045165: cell fate commitment

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-980526-332

P

Seeded From UniProt

complete


See also

References

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