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Araki, K, Kawamura, M, Suzuki, T, Matsuda, N, Kanbe, D, Ishii, K, Ichikawa, T, Kumanishi, T, Chiba, T, Tanaka, K and Nawa, H (2003) A palmitoylated RING finger ubiquitin ligase and its homologue in the brain membranes. J. Neurochem. 86:749-62


Ubiquitin (Ub) ligation is implicated in active protein metabolism and subcellular trafficking and its impairment is involved in various neurologic diseases. In rat brain, we identified two novel Ub ligases, Momo and Sakura, carrying double zinc finger motif and RING finger domain. Momo expression is enriched in the brain gray matter and testis, and Sakura expression is more widely detected in the brain white matter as well as in many peripheral organs. Both proteins associate with the cell membranes of neuronal and/or glial cells. We examined their Ub ligase activity in vivo and in vitro using viral expression vectors carrying myc-tagged Momo and Sakura. Overexpression of either Momo or Sakura in mixed cortical cultures increased total polyubiquitination levels. In vitro ubiquitination assay revealed that the combination of Momo and UbcH4 and H5c, or of Sakura and UbcH4, H5c and H6 is required for the reaction. Deletion mutagenesis suggested that the E3 Ub ligase activity of Momo and Sakura depended on their C-terminal domains containing RING finger structure, while their N-terminal domains influenced their membrane association. In agreement, Sakura associating with the membrane was specifically palmitoylated. Although the molecular targets of their Ub ligation remain to be identified, these findings imply a novel function of the palmitoylated E3 Ub ligase(s).




Amino Acid Sequence; Animals; Brain/enzymology; Cell Membrane/enzymology; Cells, Cultured; Humans; Kidney/cytology; Kidney/metabolism; Ligases/genetics; Ligases/metabolism; Male; Molecular Sequence Data; Mutagenesis, Site-Directed; Neuroglia/cytology; Neuroglia/metabolism; Neurons/cytology; Neurons/metabolism; Organ Specificity; Palmitic Acid/metabolism; Protein Structure, Tertiary/physiology; RNA, Messenger/biosynthesis; Rats; Rats, Sprague-Dawley; Sequence Homology, Amino Acid; Ubiquitin/metabolism; Ubiquitin-Protein Ligases



Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status



GO:0004842: ubiquitin-protein transferase activity

ECO:0000314: direct assay evidence used in manual assertion


Seeded From UniProt



See also


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