GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:12802074

From GONUTS
Jump to: navigation, search
Citation

Perrone, CA, Tritschler, D, Taulman, P, Bower, R, Yoder, BK and Porter, ME (2003) A novel dynein light intermediate chain colocalizes with the retrograde motor for intraflagellar transport at sites of axoneme assembly in chlamydomonas and Mammalian cells. Mol. Biol. Cell 14:2041-56

Abstract

The assembly of cilia and flagella depends on bidirectional intraflagellar transport (IFT). Anterograde IFT is driven by kinesin II, whereas retrograde IFT requires cytoplasmic dynein 1b (cDHC1b). Little is known about how cDHC1b interacts with its cargoes or how it is regulated. Recent work identified a novel dynein light intermediate chain (D2LIC) that colocalized with the mammalian cDHC1b homolog DHC2 in the centrosomal region of cultured cells. To see whether the LIC might play a role in IFT, we characterized the gene encoding the Chlamydomonas homolog of D2LIC and found its expression is up-regulated in response to deflagellation. We show that the LIC subunit copurifies with cDHC1b during flagellar isolation, dynein extraction, sucrose density centrifugation, and immunoprecipitation. Immunocytochemistry reveals that the LIC colocalizes with cDHC1b in the basal body region and along the length of flagella in wild-type cells. Localization of the complex is altered in a collection of retrograde IFT and length control mutants, which suggests that the affected gene products directly or indirectly regulate cDHC1b activity. The mammalian DHC2 and D2LIC also colocalize in the apical cytoplasm and axonemes of ciliated epithelia in the lung, brain, and efferent duct. These studies, together with the identification of an LIC mutation, xbx-1(ok279), which disrupts retrograde IFT in Caenorhabditis elegans, indicate that the novel LIC is a component of the cDHC1b/DHC2 retrograde IFT motor in a variety of organisms.

Links

PubMed PMC165096 Online version:10.1091/mbc.E02-10-0682

Keywords

Amino Acid Sequence; Animals; Chlamydomonas/genetics; Chlamydomonas/metabolism; Cytoplasmic Dyneins; Dyneins/genetics; Dyneins/metabolism; Flagella/metabolism; Molecular Motor Proteins/metabolism; Molecular Sequence Data; Rats

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CANLF:E2R699

located_in

GO:0045177: apical part of cell

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CANLF:E2R699

located_in

GO:0005929: cilium

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CANLF:E2R699

colocalizes_with

GO:0005881: cytoplasmic microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:DYL1

located_in

GO:0005930: axoneme

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:DYL1

involved_in

GO:0044458: motile cilium assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DYHC2

located_in

GO:0045177: apical part of cell

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:DYHC2

located_in

GO:0005930: axoneme

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:DYHC2

located_in

GO:0031514: motile cilium

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:IFT81

located_in

GO:0045177: apical part of cell

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:IFT81

located_in

GO:0031514: motile cilium

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:IFT81

part_of

GO:0030992: intraciliary transport particle B

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:DC2L1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9SMH5

F

Seeded From UniProt

complete

MOUSE:DC2L1

located_in

GO:0045177: apical part of cell

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:DC2L1

located_in

GO:0005930: axoneme

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:DC2L1

located_in

GO:0031514: motile cilium

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:DC2L1

colocalizes_with

GO:0036064: ciliary basal body

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q7XA07

C

Seeded From UniProt

complete

HUMAN:DC2L1

part_of

GO:0005868: cytoplasmic dynein complex

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q7XA07

C

Seeded From UniProt

complete

CHLRE:DYH1B

located_in

GO:0005930: axoneme

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:DYHC2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q7XA07

F

Seeded From UniProt

complete

CHLRE:DYHC2

enables

GO:0003774: cytoskeletal motor activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

CHLRE:DYHC2

enables

GO:0003774: motor activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

CHLRE:DYHC2

part_of

GO:0005868: cytoplasmic dynein complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:DYHC2

part_of

GO:0030990: intraciliary transport particle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:DYHC2

colocalizes_with

GO:0036064: ciliary basal body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:DYHC2

involved_in

GO:0044458: motile cilium assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHLRE:DYHC2

involved_in

GO:0072594: establishment of protein localization to organelle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHLRE:DC2L1

colocalizes_with

GO:0036064: ciliary basal body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:DC2L1

part_of

GO:0030990: intraciliary transport particle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:DC2L1

part_of

GO:0005868: cytoplasmic dynein complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:Q68RJ5

part_of

GO:0030992: intraciliary transport particle B

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:Q68RJ5

part_of

GO:0031514: motile cilium

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHLRE:Q68RJ5

part_of

GO:0045177: apical part of cell

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.