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PMID:12798298

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Citation

Mechta-Grigoriou, F, Giudicelli, F, Pujades, C, Charnay, P and Yaniv, M (2003) c-jun regulation and function in the developing hindbrain. Dev. Biol. 258:419-31

Abstract

Hindbrain development is a well-characterised segmentation process in vertebrates. The bZip transcription factor MafB/kreisler is specifically expressed in rhombomeres (r) 5 and 6 of the developing vertebrate hindbrain and is required for proper caudal hindbrain segmentation. Here, we provide evidence that the mouse protooncogene c-jun, which encodes a member of the bZip family, is coexpressed with MafB in prospective r5 and r6. Analysis of mouse mutants suggests that c-jun expression in these territories is dependent on MafB but independent of the zinc-finger transcription factor Krox20, another essential determinant of r5 development. Loss- and gain-of-function studies, performed in mouse and chick embryos, respectively, demonstrate that c-Jun participates, together with MafB and Krox20, in the transcriptional activation of the Hoxb3 gene in r5. The action of c-Jun is likely to be direct, since c-Jun homodimers and c-Jun/MafB heterodimers can bind to essential regulatory elements within the transcriptional enhancer responsible for Hoxb3 expression in r5. These data indicate that c-Jun acts both as a downstream effector and a cofactor of MafB and belongs to the complex network of factors governing hindbrain patterning.

Links

PubMed

Keywords

Animals; Avian Proteins; Binding Sites/genetics; Chick Embryo; DNA-Binding Proteins/metabolism; Early Growth Response Protein 2; Enhancer Elements, Genetic; Female; Gene Expression Regulation, Developmental; Genes, jun; Homeodomain Proteins/genetics; In Situ Hybridization; MafB Transcription Factor; Mice; Mice, Inbred C57BL; Mice, Mutant Strains; Mutation; Oncogene Proteins/metabolism; Pregnancy; Rhombencephalon/embryology; Rhombencephalon/metabolism; Trans-Activators/metabolism; Transcription Factors/metabolism; Transcriptional Activation; Xenopus Proteins/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:JUN

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P54841

F

Seeded From UniProt

complete

MOUSE:JUN

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:JUN

part_of

GO:0005667: transcription regulator complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:JUN

acts_upstream_of_or_within

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

  • has_input:(MGI:MGI:96184)

Seeded From UniProt

complete

MOUSE:MAFB

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P05627

F

Seeded From UniProt

complete

MOUSE:MAFB

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:MAFB

part_of

GO:0005667: transcription regulator complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:MAFB

acts_upstream_of_or_within

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

  • has_input:(MGI:MGI:96184)

Seeded From UniProt

complete


See also

References

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