PMID:12783785

Citation	Lin, HV, Doroquez, DB, Cho, S, Chen, F, Rebay, I and Cadigan, KM (2003) Splits ends is a tissue/promoter specific regulator of Wingless signaling. Development 130:3125-35
Abstract	Wingless directs many developmental processes in Drosophila by regulating expression of specific target genes through a conserved signaling pathway. Although many nuclear factors have been implicated in mediating Wingless-induced transcription, the mechanism of how Wingless regulates different targets in different tissues remains poorly understood. We report here that the split ends gene is required for Wingless signaling in the eye, wing and leg imaginal discs. Expression of a dominant-negative version of split ends resulted in more dramatic reductions in Wingless signaling than split ends-null alleles, suggesting that it may have a redundant partner. However, removal of split ends or expression of the dominant-negative had no effect on several Wingless signaling readouts in the embryo. The expression pattern of Split ends cannot explain this tissue-specific requirement, as the protein is predominantly nuclear and present throughout embryogenesis and larval tissues. Consistent with its nuclear location, the split ends dominant-negative acts downstream of Armadillo stabilization. Our data indicate that Split ends is an important positive regulator of Wingless signaling in larval tissues. However, it has no detectable role in the embryonic Wingless pathway, suggesting that it is a tissue or promoter-specific factor.
Links	PubMed
Keywords	Alleles; Animals; Cell Nucleus/metabolism; Drosophila Proteins/biosynthesis; Drosophila Proteins/genetics; Drosophila melanogaster; Genes, Dominant; Homeodomain Proteins/genetics; Homeodomain Proteins/physiology; Microscopy, Electron, Scanning; Microscopy, Fluorescence; Mutation; Nuclear Proteins/genetics; Nuclear Proteins/physiology; Phenotype; Photoreceptor Cells, Invertebrate/pathology; Photoreceptor Cells, Invertebrate/ultrastructure; Promoter Regions, Genetic; Proto-Oncogene Proteins/biosynthesis; Proto-Oncogene Proteins/genetics; Signal Transduction; Wnt1 Protein
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
DROME:SPEN	located_in	GO:0005634: nucleus	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
DROME:SPEN	involved_in	GO:0090263: positive regulation of canonical Wnt signaling pathway	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.