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PMID:12766090
| Citation |
Stamer, WD, Bok, D, Hu, J, Jaffe, GJ and McKay, BS (2003) Aquaporin-1 channels in human retinal pigment epithelium: role in transepithelial water movement. Invest. Ophthalmol. Vis. Sci. 44:2803-8 |
|---|---|
| Abstract |
Aquaporin (AQP) is a hexahelical integral membrane protein that functions as a constitutive channel for water and regulated channel for cations in fluid transporting tissues, including many in the eye. Although AQP1 has been cloned from a cDNA library prepared from cultures of retinal pigment epithelial (RPE) cells isolated from human fetal tissue, three separate studies failed with various immunochemical techniques to detect AQP1 protein in adult human or rat RPE preparations. The purpose of this study was to examine specifically the expression and distribution of AQP1 in adult human RPE in situ by using alternative methodologies and model systems and to determine the contribution of AQP1 to water movement across cultured RPE cells isolated from human cadaveric and fetal eyes. |
| Links | |
| Keywords |
Adenoviridae/genetics; Adolescent; Aquaporin 1; Aquaporins/genetics; Aquaporins/metabolism; Biological Transport; Biotin; Blood Group Antigens; Cell Membrane Permeability; Cells, Cultured; Electrophoresis, Polyacrylamide Gel; Fetus; Fluorescent Antibody Technique, Indirect; Gene Expression Regulation; Genetic Vectors; Humans; Microscopy, Confocal; Middle Aged; Pigment Epithelium of Eye/metabolism; Water/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
located_in |
GO:0016323: basolateral plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
located_in |
GO:0016324: apical plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016324: apical plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006833: water transport |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005372: water transmembrane transporter activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0035377: transepithelial water transport |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016323: basolateral plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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