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PMID:12766090

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Citation

Stamer, WD, Bok, D, Hu, J, Jaffe, GJ and McKay, BS (2003) Aquaporin-1 channels in human retinal pigment epithelium: role in transepithelial water movement. Invest. Ophthalmol. Vis. Sci. 44:2803-8

Abstract

Aquaporin (AQP) is a hexahelical integral membrane protein that functions as a constitutive channel for water and regulated channel for cations in fluid transporting tissues, including many in the eye. Although AQP1 has been cloned from a cDNA library prepared from cultures of retinal pigment epithelial (RPE) cells isolated from human fetal tissue, three separate studies failed with various immunochemical techniques to detect AQP1 protein in adult human or rat RPE preparations. The purpose of this study was to examine specifically the expression and distribution of AQP1 in adult human RPE in situ by using alternative methodologies and model systems and to determine the contribution of AQP1 to water movement across cultured RPE cells isolated from human cadaveric and fetal eyes.

Links

PubMed

Keywords

Adenoviridae/genetics; Adolescent; Aquaporin 1; Aquaporins/genetics; Aquaporins/metabolism; Biological Transport; Biotin; Blood Group Antigens; Cell Membrane Permeability; Cells, Cultured; Electrophoresis, Polyacrylamide Gel; Fetus; Fluorescent Antibody Technique, Indirect; Gene Expression Regulation; Genetic Vectors; Humans; Microscopy, Confocal; Middle Aged; Pigment Epithelium of Eye/metabolism; Water/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:AQP1

located_in

GO:0016323: basolateral plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:AQP1

located_in

GO:0016324: apical plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:AQP1

part_of

GO:0016324: apical plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:AQP1

involved_in

GO:0006833: water transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:AQP1

enables

GO:0005372: water transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:AQP1

involved_in

GO:0035377: transepithelial water transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:AQP1

part_of

GO:0016323: basolateral plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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