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PMID:12723693

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Citation

Fanti, L, Berloco, M, Piacentini, L and Pimpinelli, S (2003) Chromosomal distribution of heterochromatin protein 1 (HP1) in Drosophila: a cytological map of euchromatic HP1 binding sites. Genetica 117:135-47

Abstract

The Heterochromatin Protein 1 (HP1) is a conserved protein which is best known for its strong association with the heterochromatin of Drosophila melanogaster. We previously demonstrated that another important property of HP1 is its localization to the telomeres of Drosophila, a feature that reflects its critical function as a telomere capping protein. Here we report our analysis of the euchromatic sites to which HP1 localizes. Using an anti-HP1 antibody, we compared immunostaining patterns on polytene chromosomes of the Ore-R wild type laboratory strain and four different natural populations. HP1 was found to accumulate at specific euchromatic sites, with a subset of the sites conserved among strains. These sites do not appear to be defined by an enrichment of known repetitive DNAs. Comparisons of HP1 patterns among several Drosophila species revealed that association with specific euchromatic regions, heterochromatin and telomeres is a conserved characteristic of HP1. Based on these results, we argue that HP1 serves a broader function than typically postulated. In addition to its role in heterochromatin assembly and telomere stability, we propose that HP1 plays an important role in regulating the expression of many different euchromatic regions.

Links

PubMed

Keywords

Animals; Chromosomal Proteins, Non-Histone/genetics; Chromosome Mapping; Drosophila Proteins/genetics; Drosophila melanogaster/genetics; Euchromatin/genetics; Fluorescent Antibody Technique; Heterochromatin/genetics; In Situ Hybridization, Fluorescence

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:HP1

located_in

GO:0010369: chromocenter

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:HP1

located_in

GO:0000791: euchromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:HP1

located_in

GO:0000781: chromosome, telomeric region

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:HP1

part_of

GO:0005701: polytene chromosome chromocenter

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:HP1

part_of

GO:0035012: polytene chromosome, telomeric region

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:HP1

part_of

GO:0005703: polytene chromosome puff

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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