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PMID:12715897

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Citation

Missirlis, F, Rahlfs, S, Dimopoulos, N, Bauer, H, Becker, K, Hilliker, A, Phillips, JP and Jäckle, H (2003) A putative glutathione peroxidase of Drosophila encodes a thioredoxin peroxidase that provides resistance against oxidative stress but fails to complement a lack of catalase activity. Biol. Chem. 384:463-72

Abstract

Cellular defense systems against reactive oxygen species (ROS) include thioredoxin reductase (TrxR) and glutathione reductase (GR). They generate sulfhydryl-reducing systems which are coupled to antioxidant enzymes, the thioredoxin and glutathione peroxidases (TPx and GPx). The fruit fly Drosophila lacks a functional GR, suggesting that the thioredoxin system is the major source for recycling glutathione. Whole genome in silico analysis identified two non-selenium containing putative GPx genes. We examined the biochemical characteristics of one of these gene products and found that it lacks GPx activity and functions as a TPx. Transgene-dependent overexpression of the newly identified Glutathione peroxidase homolog with thioredoxin peroxidase activity (Gtpx-1) gene increases resistance to experimentally induced oxidative stress, but does not compensate for the loss of catalase, an enzyme which, like GTPx-1, functions to eliminate hydrogen peroxide. The results suggest that GTPx-1 is part of the Drosophila Trx antioxidant defense system but acts in a genetically distinct pathway or in a different cellular compartment than catalase.

Links

PubMed Online version:10.1515/BC.2003.052

Keywords

Animals; Animals, Genetically Modified; Base Sequence; Catalase/genetics; Catalase/metabolism; Drosophila melanogaster/enzymology; Drosophila melanogaster/genetics; Drosophila melanogaster/physiology; Gene Expression/drug effects; Gene Expression/physiology; Genes, Insect/genetics; Glutathione Peroxidase/genetics; Hyperoxia/physiopathology; In Situ Hybridization; Longevity/genetics; Molecular Sequence Data; Neoplasm Proteins; Oxidative Stress/drug effects; Oxidative Stress/physiology; Paraquat/toxicity; Peroxidases/genetics; Peroxiredoxins; Reactive Oxygen Species/metabolism; Sequence Alignment; Transgenes/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:Q8IRD3

involved_in

GO:0006979: response to oxidative stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8IRD3

located_in

GO:0005794: Golgi apparatus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q8IRD4

involved_in

GO:0006979: response to oxidative stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8IRD4

located_in

GO:0005794: Golgi apparatus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VZQ8

involved_in

GO:0006979: response to oxidative stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VZQ8

located_in

GO:0005794: Golgi apparatus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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