GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:12682017

From GONUTS
Jump to: navigation, search
Citation

Simic, R, Lindstrom, DL, Tran, HG, Roinick, KL, Costa, PJ, Johnson, AD, Hartzog, GA and Arndt, KM (2003) Chromatin remodeling protein Chd1 interacts with transcription elongation factors and localizes to transcribed genes. EMBO J. 22:1846-56

Abstract

Transcription in eukaryotes is influenced by the chromatin state of the template, and chromatin remodeling factors have well-documented roles in regulating transcription initiation by RNA polymerase (pol) II. Chromatin also influences transcription elongation; however, little is known about the role of chromatin remodeling factors in this process. Here, we present evidence that the Saccharomyces cerevisiae chromatin remodeling factor Chd1 functions during transcription elongation. First, we identified Chd1 in a two-hybrid screen for proteins that interact with Rtf1, a member of the Paf1 complex that associates with RNA pol II and regulates transcription elongation. Secondly, we show through co-immunoprecipitation studies that Chd1 also interacts with components of two essential elongation factors, Spt4-Spt5 and Spt16-Pob3. Thirdly, we demonstrate that deletion of CHD1 suppresses a cold-sensitive spt5 mutation that is also suppressed by defects in the Paf1 complex and RNA pol II. Finally, we demonstrate that Chd1, Rtf1 and Spt5 associate with actively transcribed regions of chromatin. Collectively, these findings suggest an important role for Chd1 and chromatin remodeling in the control of transcription elongation.

Links

PubMed PMC154471 Online version:10.1093/emboj/cdg179

Keywords

Chromatin/metabolism; Chromosomal Proteins, Non-Histone; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; TATA-Box Binding Protein/metabolism; Transcription Factors/metabolism; Transcription, Genetic; Transcriptional Elongation Factors/metabolism; Two-Hybrid System Techniques

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:CHD1

located_in

GO:0000785: chromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:CHD1

enables

GO:0031490: chromatin DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:CHD1

involved_in

GO:0006368: transcription elongation from RNA polymerase II promoter

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000004470

P

Seeded From UniProt

complete

YEAST:CHD1

involved_in

GO:0006368: transcription elongation from RNA polymerase II promoter

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000003213

P

Seeded From UniProt

complete

YEAST:CHD1

involved_in

GO:0006368: transcription elongation from RNA polymerase II promoter

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000004470

P

Seeded From UniProt

complete

YEAST:CHD1

involved_in

GO:0006368: transcription elongation from RNA polymerase II promoter

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000004534

P

Seeded From UniProt

complete

YEAST:RTF1

located_in

GO:0035327: transcriptionally active chromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.