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PMID:12681493

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Citation

Fujii, Y, Wakahara, S, Nakao, T, Hara, T, Ohtake, H, Komurasaki, T, Kitamura, K, Tatsuno, A, Fujiwara, N, Hozumi, N, Ra, C, Kitamura, D and Goitsuka, R (2003) Targeting of MIST to Src-family kinases via SKAP55-SLAP-130 adaptor complex in mast cells. FEBS Lett. 540:111-6

Abstract

MIST (mast cell immunoreceptor signal transducer; also termed Clnk) is an adaptor protein structurally related to SLP-76-family hematopoietic cell-specific adaptor proteins. We demonstrate here that two major MIST-associated phosphoproteins expressed in mast cell lines are SLAP-130 and SKAP55, adaptors known to interact with the Src-homology (SH) 2 domain of Src-family protein tyrosine kinases (PTKs). MIST directly associated with SLAP-130 via its SH2 domain, and collaboration of SLAP-130 with SKAP55 was required for the recruitment of MIST to Lyn. Furthermore, MIST was preferentially recruited to Fyn rather than Lyn, which is regulated by higher affinity binding of SLAP-130 and SKAP55 with the Fyn-SH2 domain than the Lyn-SH2 domain. Our results suggest that the MIST-SLAP-130-SKAP55 adaptor complex functions downstream of high-affinity IgE receptor-associated Src-PTKs in mast cells.

Links

PubMed

Keywords

Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Animals; Carrier Proteins/chemistry; Carrier Proteins/metabolism; Humans; Mast Cells/enzymology; Mast Cells/metabolism; Mice; Molecular Sequence Data; Phosphoproteins/chemistry; Phosphoproteins/metabolism; Rats; Sequence Homology, Amino Acid; src-Family Kinases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:FYB1

enables

GO:0044877: protein-containing complex binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:1557394
RGD:621017
RGD:708436

F

Seeded From UniProt

complete

MOUSE:FYB1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P25911,UniProtKB:Q4V7G1,UniProtKB:Q9QZE2

F

Seeded From UniProt

complete

MOUSE:FYB1

part_of

GO:0032991: protein-containing complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:LYN

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O35601,UniProtKB:Q4V7G1,UniProtKB:Q9QZE2

F

Seeded From UniProt

complete

MOUSE:LYN

involved_in

GO:0018108: peptidyl-tyrosine phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:LYN

enables

GO:0004713: protein tyrosine kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:LYN

part_of

GO:0032991: protein-containing complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:FYN

involved_in

GO:0018108: peptidyl-tyrosine phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:FYN

enables

GO:0004713: protein tyrosine kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

RAT:LYN

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:1562417

F

Seeded From UniProt

complete

RAT:SKAP1

enables

GO:0042169: SH2 domain binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:1557394

F

Seeded From UniProt

complete

RAT:SKAP1

enables

GO:0044877: protein-containing complex binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:1557394
RGD:1563421
RGD:621017

F

Seeded From UniProt

complete

RAT:SKAP1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O35601,UniProtKB:P25911,UniProtKB:Q9QZE2

F

Seeded From UniProt

complete

RAT:SKAP1

part_of

GO:0032991: protein-containing complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:CLNK

part_of

GO:0032991: protein-containing complex

ECO:0000315: mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:CLNK

enables

GO:0044877: protein-containing complex binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O35601,UniProtKB:P25911,UniProtKB:Q4V7G1

F

Seeded From UniProt

complete

RAT:LYN

enables

GO:0044877: protein-containing complex binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:1557394
RGD:1563421
RGD:708436

F

Seeded From UniProt

complete

Notes

See also

References

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