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PMID:12653554

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Citation

Stemmer, C, Leeming, DJ, Franssen, L, Grimm, R and Grasser, KD (2003) Phosphorylation of maize and Arabidopsis HMGB proteins by protein kinase CK2alpha. Biochemistry 42:3503-8

Abstract

In plants, a variety of chromatin-associated high mobility group (HMG) proteins belonging to the HMGB family have been identified. We have examined the phosphorylation of the HMGB proteins from the monocotyledonous plant maize and the dicotyledonous plant Arabidopsis by protein kinase CK2alpha. Maize CK2alpha phosphorylates the maize HMGB1 and HMGB2/3 proteins and the Arabidopsis HMGB1, HMGB2/3, and HMGB4 proteins. Maize HMGB4 and HMGB5 and Arabidopsis HMGB5 are not phosphorylated by CK2alpha. Depending on the HMGB protein up to five amino acid residues are phosphorylated in the course of the phosphorylation reaction. The HMGB1 proteins from both plants are markedly more slowly phosphorylated by CK2alpha than the other HMGB substrate proteins, indicating that certain HMGB proteins are clearly preferred substrates for CK2alpha. The rate of the phosphorylation reaction appears to be related to the ease of interaction between CK2alpha and the HMGB proteins, as indicated by chemical cross-linking experiments. MALDI/TOF mass spectrometry analyses demonstrate that the HMGB1 and HMGB2/3 proteins occur in various phosphorylation states in immature maize kernels. Thus, HMGB1 exists as monophosphorylated, double-phosphorylated, triple-phosphorylated, and tetraphosphorylated protein in kernel tissue, and the tetraphosphorylated form is the most abundant version. The observed in vivo phosphorylation states indicate that protein kinase(s) other than CK2alpha contribute(s) to the modification of the plant HMGB proteins. The fact that the HMGB proteins are phosphorylated to various extents reveals that the existence of differentially modified forms increases the number of distinct HMGB protein variants in plant chromatin that may be adapted to certain functions.

Links

PubMed Online version:10.1021/bi027350d

Keywords

Arabidopsis/metabolism; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/metabolism; Casein Kinase II; HMGB Proteins/chemistry; HMGB Proteins/metabolism; HMGB1 Protein/chemistry; HMGB1 Protein/metabolism; HMGB2 Protein/chemistry; HMGB2 Protein/metabolism; HMGB3 Protein/chemistry; HMGB3 Protein/metabolism; Kinetics; Phosphorylation; Plant Proteins/chemistry; Plant Proteins/metabolism; Protein-Serine-Threonine Kinases/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Zea mays/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:HMGB1

located_in

GO:0000785: chromatin

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

ARATH:HMGB1

acts_upstream_of_or_within

GO:0006333: chromatin assembly or disassembly

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ARATH:HMGB1

enables

GO:0030527: structural constituent of chromatin

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

ARATH:HMGB1

enables

GO:0003682: chromatin binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

ARATH:HMGB2

located_in

GO:0000785: chromatin

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

ARATH:HMGB2

acts_upstream_of_or_within

GO:0006333: chromatin assembly or disassembly

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ARATH:HMGB2

enables

GO:0030527: structural constituent of chromatin

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

ARATH:HMGB2

enables

GO:0003682: chromatin binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

ARATH:HMGB5

located_in

GO:0000785: chromatin

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

ARATH:HMGB5

acts_upstream_of_or_within

GO:0006333: chromatin assembly or disassembly

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ARATH:HMGB5

enables

GO:0030527: structural constituent of chromatin

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

ARATH:HMGB5

enables

GO:0003682: chromatin binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

ARATH:HMGB3

located_in

GO:0000785: chromatin

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

ARATH:HMGB3

acts_upstream_of_or_within

GO:0006333: chromatin assembly or disassembly

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ARATH:HMGB3

enables

GO:0030527: structural constituent of chromatin

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

ARATH:HMGB3

enables

GO:0003682: chromatin binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

ARATH:HMGB4

located_in

GO:0000785: chromatin

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

ARATH:HMGB4

acts_upstream_of_or_within

GO:0006333: chromatin assembly or disassembly

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ARATH:HMGB4

enables

GO:0030527: structural constituent of chromatin

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

ARATH:HMGB4

enables

GO:0003682: chromatin binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete


See also

References

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