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PMID:12641734

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Citation

Yap, CC, Liang, F, Yamazaki, Y, Muto, Y, Kishida, H, Hayashida, T, Hashikawa, T and Yano, R (2003) CIP98, a novel PDZ domain protein, is expressed in the central nervous system and interacts with calmodulin-dependent serine kinase. J. Neurochem. 85:123-34

Abstract

Receptors and various molecules in neurons are localized at precise locations to perform their respective functions, especially in synaptic sites. Among synaptic molecules, PDZ domain proteins play major roles in scaffolding and anchoring membrane proteins for efficient synaptic transmission. In the present study, we isolated CIP98, a novel protein (98 kDa) consisting of three PDZ domains and a proline-rich region, which is widely expressed in the central nervous system. In situ hybridization and immunohistochemical staining patterns demonstrate that CIP98 is expressed strongly in certain types of neurons, i.e. pyramidal cells in layers III-V of the cerebral cortex, projecting neurons in the thalamus and interneurons in the cerebellum. The results of immunocytochemical staining and electron microscopy revealed that CIP98 is localized both in dendrites and axons. Interestingly, CIP98 interacts with CASK (calmodulin-dependent serine kinase), a member of the membrane-associated guanylate kinase (MAGUK) family that plays important roles in the molecular organization of proteins at synapses. CIP98 was shown to co-localize with CASK along the dendritic processes of neurons. In view of its direct association with CASK, CIP98 may be involved in the formation of CASK scaffolding proteins complex to facilitate synaptic transmission in the CNS.

Links

PubMed

Keywords

Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Animals; Calcium-Calmodulin-Dependent Protein Kinases; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cells, Cultured; Central Nervous System/metabolism; Green Fluorescent Proteins; Guanylate Kinase; Humans; In Situ Hybridization; Luminescent Proteins/genetics; Male; Molecular Sequence Data; Nerve Tissue Proteins/chemistry; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Neurons/cytology; Neurons/metabolism; Nucleoside-Phosphate Kinase/genetics; Nucleoside-Phosphate Kinase/metabolism; Organ Specificity; Protein Binding/physiology; Protein Structure, Tertiary/physiology; Rats; Rats, Wistar; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Sequence Homology, Amino Acid; Two-Hybrid System Techniques

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:CSKP

located_in

GO:0048471: perinuclear region of cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CSKP

located_in

GO:0014069: postsynaptic density

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CSKP

located_in

GO:0030425: dendrite

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:WHRN

enables

GO:0019904: protein domain specific binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:62004

F

Seeded From UniProt

complete

RAT:WHRN

located_in

GO:0044303: axon collateral

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:WHRN

located_in

GO:0048471: perinuclear region of cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:WHRN

located_in

GO:0098794: postsynapse

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:WHRN

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:WHRN

located_in

GO:0098793: presynapse

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:WHRN

located_in

GO:0043198: dendritic shaft

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:WHRN

located_in

GO:0097440: apical dendrite

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:WHRN

located_in

GO:0043025: neuronal cell body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CSKP

enables

GO:0030165: PDZ domain binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:631330

F

Seeded From UniProt

complete

RAT:CSKP

part_of

GO:0030425: dendrite

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CSKP

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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