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PMID:12619136

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Citation

Cui, S, Schwartz, L and Quaggin, SE (2003) Pod1 is required in stromal cells for glomerulogenesis. Dev. Dyn. 226:512-22

Abstract

Pod1 (capsulin/epicardin/Tcf21) is a basic-helix-loop-helix transcription factor that is highly expressed in the mesenchyme of developing organs that include the kidney, lung, gut, and heart. Null Pod1 mice are born but die shortly after birth due to a lack of alveoli in the lungs and cardiac defects. In addition, the kidneys are hypoplastic and demonstrate disrupted branching morphogenesis of the ureteric bud epithelium, a marked reduction in the number of nephrons, a delay in glomerulogenesis, and blood vessel abnormalities. To further dissect the cellular function of Pod1 during kidney development, chimeric mice were generated through aggregations of null Pod1 embryonic stem cells and murine embryos ubiquitously expressing enhanced green fluorescent protein (GFP). Histologic, immunohistochemical, and in situ hybridization analysis of the resulting chimeric offspring demonstrated both cell autonomous and non-cell autonomous roles for Pod1 in the differentiation of specific renal cell lineages that include peritubular interstitial cells and pericytes. Most strikingly, the glomerulogenesis defect was rescued by the presence of wild-type stromal cells, suggesting a non-cell autonomous role for Pod1 in this cell population.

Links

PubMed Online version:10.1002/dvdy.10244

Keywords

Animals; Basic Helix-Loop-Helix Transcription Factors; Capillaries/cytology; Capillaries/embryology; Cell Differentiation/physiology; Cell Lineage/physiology; Epithelial Cells/cytology; Epithelial Cells/physiology; Gene Expression Regulation, Developmental; Kidney Glomerulus/cytology; Kidney Glomerulus/embryology; Kidney Glomerulus/physiology; Mesoderm/cytology; Mesoderm/physiology; Mice; Mice, Mutant Strains; Nephrons/cytology; Nephrons/embryology; Recombinant Fusion Proteins; Renal Circulation/physiology; Stromal Cells/cytology; Stromal Cells/physiology; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:TCF21

involved_in

GO:0072277: metanephric glomerular capillary formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:TCF21

involved_in

GO:0072162: metanephric mesenchymal cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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