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PMID:12600943

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Citation

Sharma, VM, Li, B and Reese, JC (2003) SWI/SNF-dependent chromatin remodeling of RNR3 requires TAF(II)s and the general transcription machinery. Genes Dev. 17:502-15

Abstract

Gene expression requires the recruitment of chromatin remodeling activities and general transcription factors (GTFs) to promoters. Whereas the role of activators in recruiting chromatin remodeling activities has been clearly demonstrated, the contributions of the transcription machinery have not been firmly established. Here we demonstrate that the remodeling of the RNR3 promoter requires a number of GTFs, mediator and RNA polymerase II. We also show that remodeling is dependent upon the SWI/SNF complex, and that TFIID and RNA polymerase II are required for its recruitment to the promoter. In contrast, Gcn5p-dependent histone acetylation occurs independently of TFIID and RNA polymerase II function, and we provide evidence that acetylation increases the extent of nucleosome remodeling, but is not required for SWI/SNF recruitment. Thus, the general transcription machinery can contribute to nucleosome remodeling by mediating the association of SWI/SNF with promoters, thereby revealing a novel pathway for the recruitment of chromatin remodeling activities.

Links

PubMed PMC195993 Online version:10.1101/gad.1039503

Keywords

Acetylation; Chromatin/genetics; Chromatin/metabolism; Cyclin-Dependent Kinases/genetics; Cyclin-Dependent Kinases/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Drosophila Proteins; Fungal Proteins/genetics; Fungal Proteins/metabolism; Histones/metabolism; Mediator Complex; Mutation; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Nucleosomes/metabolism; Promoter Regions, Genetic; Protein Kinases/genetics; Protein Kinases/metabolism; RNA Polymerase II/genetics; RNA Polymerase II/metabolism; RNA-Binding Proteins; Ribonucleoprotein, U1 Small Nuclear/genetics; Ribonucleoprotein, U1 Small Nuclear/metabolism; Ribonucleotide Reductases/genetics; Ribonucleotide Reductases/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; TATA-Binding Protein Associated Factors/genetics; TATA-Binding Protein Associated Factors/metabolism; Transcription Factor TFIID/genetics; Transcription Factor TFIID/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic; Yeasts/genetics; Yeasts/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:TAF2

enables

GO:0003682: chromatin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:TAF5

enables

GO:0003682: chromatin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:TAF1

enables

GO:0003682: chromatin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:TAF1

involved_in

GO:0051123: RNA polymerase II preinitiation complex assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:TAF4

enables

GO:0003682: chromatin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:TAF12

enables

GO:0003682: chromatin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:TAF12

involved_in

GO:0051123: RNA polymerase II preinitiation complex assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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