PMID:12538856

Citation	Taylor, NG, Howells, RM, Huttly, AK, Vickers, K and Turner, SR (2003) Interactions among three distinct CesA proteins essential for cellulose synthesis. Proc. Natl. Acad. Sci. U.S.A. 100:1450-5
Abstract	In a screen to identify novel cellulose deficient mutants, three lines were shown to be allelic and define a novel complementation group, irregular xylem5 (irx5). IRX5 was cloned and encodes a member of the CesA family of cellulose synthase catalytic subunits (AtCesA4). irx5 plants have an identical phenotype to previously described mutations in two other members of this gene family (IRX1 and IRX3). IRX5, IRX3, and IRX1 are coexpressed in exactly the same cells, and all three proteins interact in detergent solubilized extracts, suggesting that three members of this gene family are required for cellulose synthesis in secondary cell walls. The association of IRX1 and IRX3 was reduced to undetectable levels in the absence of IRX5. Consequently, these data suggest that IRX5, IRX3, and IRX1 are all essential components of the cellulose synthesizing complex and the presence of all three subunits is required for the correct assembly of this complex.
Links	PubMed PMC298793 Online version:10.1073/pnas.0337628100
Keywords	Arabidopsis/genetics; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cell Wall/metabolism; Cellulose/biosynthesis; Glucosyltransferases/genetics; Glucosyltransferases/metabolism; Homeodomain Proteins/genetics; Homeodomain Proteins/metabolism; Molecular Sequence Data; Phenotype; Polymerase Chain Reaction; Protein Binding; Protein Structure, Tertiary; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Temperature; Transcription Factors/genetics; Transcription Factors/metabolism
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Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
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