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PMID:12527778

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Citation

Chen, W, Bucaria, J, Band, DA, Sutton, A and Sternglanz, R (2003) Enp1, a yeast protein associated with U3 and U14 snoRNAs, is required for pre-rRNA processing and 40S subunit synthesis. Nucleic Acids Res. 31:690-9

Abstract

ENP1 is an essential Saccharomyces cerevisiae gene encoding a 483 amino acid polypeptide. Enp1 protein is localized in the nucleus and concentrated in the nucleolus. An enp1-1 temperature-sensitive mutant inhibited 35S pre-rRNA early processing at sites A(0), A(1) and A(2) as shown by northern analysis of steady state levels of rRNA precursors. Pulse-chase analysis further revealed that the enp1-1 strain was defective in the synthesis of 20S pre-rRNA and hence 18S rRNA, which led to reduced formation of 40S ribosomal subunits. Co-precipitation analysis revealed that Enp1 was associated with Nop1 protein, as well as with U3 and U14 RNAs, two snoRNAs implicated in early pre-rRNA processing steps. These results suggest a direct role for Enp1 in the early steps of rRNA processing.

Links

PubMed PMC140510

Keywords

Alleles; Amino Acid Sequence; Binding Sites; Cell Nucleolus/metabolism; Green Fluorescent Proteins; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Molecular Sequence Data; Mutation; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Protein Binding; RNA Precursors/genetics; RNA Precursors/metabolism; RNA Processing, Post-Transcriptional; RNA, Small Nucleolar/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Ribonucleoproteins, Small Nucleolar/metabolism; Ribosomes/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Sequence Homology, Amino Acid; Temperature

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:FBRL

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P38333

F

Seeded From UniProt

complete

YEAST:ENP1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P15646

F

Seeded From UniProt

complete

YEAST:ENP1

involved_in

GO:0006364: rRNA processing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:ENP1

involved_in

GO:0006364: rRNA processing

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000002172

P

Seeded From UniProt

complete

YEAST:ENP1

enables

GO:0030515: snoRNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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