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PMID:12514262

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Citation

Bertinato, J, Iskandar, M and L'Abbé, MR (2003) Copper deficiency induces the upregulation of the copper chaperone for Cu/Zn superoxide dismutase in weanling male rats. J. Nutr. 133:28-31

Abstract

The most commonly used indices for determining copper deficiency in humans are reduced serum/plasma copper concentration and decreased activity of ceruloplasmin and Cu/Zn superoxide dismutase (SOD1). However, these indicators are influenced by many factors unrelated to copper status and lack the sensitivity required to detect marginal deficiency, limiting their usefulness in many situations. In vivo, the insertion of copper into SOD1 is dependent on the copper chaperone for SOD1 (CCS). In this study, we explored the possibility that the expression level of CCS may reflect copper status and thus serve as a useful marker of copper nutriture. Weanling male Wistar rats were fed either a normal (5.3 mg Cu/kg diet), moderately deficient (0.84 mg Cu/kg diet) or deficient (0.34 mg Cu/kg diet) copper diet for 6 wk. Rats fed moderate and deficient diets showed differences (P < 0.05) in several hematological measurements, indicating varying degrees of copper deficiency in these groups. Copper-deficient rats had reduced (P < 0.05) liver and erythrocyte SOD1 activity and body weight. Western blot analysis revealed a dose-dependent increase (P < 0.05) in CCS expression in liver and erythrocytes of copper-deficient rats. We report CCS protein level as a novel marker for assessing copper status.

Links

PubMed

Keywords

Animals; Arabidopsis Proteins; Copper/administration & dosage; Copper/deficiency; Copper/pharmacology; Diet; Liver/drug effects; Liver/enzymology; Male; Molecular Chaperones/metabolism; Rats; Rats, Wistar; Superoxide Dismutase/metabolism; Up-Regulation/drug effects; Up-Regulation/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:CCS

enables

GO:0016532: superoxide dismutase copper chaperone activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

RAT:SODC

enables

GO:0004784: superoxide dismutase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

RAT:SODC

enables

GO:0005507: copper ion binding

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

RAT:SODC

involved_in

GO:0006801: superoxide metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:SODC

involved_in

GO:0019430: removal of superoxide radicals

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:SODC

involved_in

GO:0046688: response to copper ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:SODC

involved_in

GO:0050665: hydrogen peroxide biosynthetic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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