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PMID:12504018

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Citation

Alén, C, Kent, NA, Jones, HS, O'Sullivan, J, Aranda, A and Proudfoot, NJ (2002) A role for chromatin remodeling in transcriptional termination by RNA polymerase II. Mol. Cell 10:1441-52

Abstract

Chromatin remodeling can facilitate the recruitment of RNA polymerase II (Pol II) to targeted promoters, as well as enhancing the level of transcription. Here, we describe a further key role for chromatin remodeling in transcriptional termination. Using a genetic screen in S. pombe, we identified the CHD-Mi2 class chromatin remodeling ATPase, Hrp1, as a termination factor. In S. cerevisiae, we show that transcriptional termination and chromatin structure at the 3' ends of three genes all depend on the activity of the Hrp1 homolog, Chd1p, either alone or redundantly with the ISWI ATPases, Isw1p, and Isw2p. We suggest that chromatin remodeling of termination regions is a necessary prelude to efficient Pol II termination.

Links

PubMed

Keywords

3' Untranslated Regions/genetics; Binding Sites; Chromatin/genetics; Chromatin/ultrastructure; DNA, Fungal/genetics; DNA, Fungal/metabolism; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Fungal; Micrococcal Nuclease/metabolism; RNA Polymerase II/genetics; RNA Polymerase II/metabolism; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Schizosaccharomyces/enzymology; Schizosaccharomyces/genetics; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:CHD1

located_in

GO:0000785: chromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:CHD1

involved_in

GO:0006369: termination of RNA polymerase II transcription

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:CHD1

involved_in

GO:0006369: termination of RNA polymerase II transcription

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000449
SGD:S000005831

P

Seeded From UniProt

complete

YEAST:ISW1

involved_in

GO:0006369: termination of RNA polymerase II transcription

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000966
SGD:S000005831

P

Seeded From UniProt

complete

YEAST:ISW2

involved_in

GO:0006369: termination of RNA polymerase II transcription

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000449
SGD:S000000966

P

Seeded From UniProt

complete


See also

References

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