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PMID:12482982

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Citation

Marenda, DR, Zraly, CB, Feng, Y, Egan, S and Dingwall, AK (2003) The Drosophila SNR1 (SNF5/INI1) subunit directs essential developmental functions of the Brahma chromatin remodeling complex. Mol. Cell. Biol. 23:289-305

Abstract

The Drosophila melanogaster Brahma (Brm) complex, a counterpart of the Saccharomyces cerevisiae SWI/SNF ATP-dependent chromatin remodeling complex, is important for proper development by maintaining specific gene expression patterns. The SNR1 subunit is strongly conserved with yeast SNF5 and mammalian INI1 and is required for full activity of the Brm complex. We identified a temperature-sensitive allele of snr1 caused by a single amino acid substitution in the conserved repeat 2 region, implicated in a variety of protein-protein interactions. Genetic analyses of snr1(E1) reveal that it functions as an antimorph and that snr1 has critical roles in tissue patterning and growth control. Temperature shifts show that snr1 is continuously required, with essential functions in embryogenesis, pupal stages, and adults. Allele-specific genetic interactions between snr1(E1) and mutations in genes encoding other members of the Brm complex suggest that snr1(E1) mutant phenotypes result from reduced Brm complex function. Consistent with this view, SNR1(E1) is stably associated with other components of the Brm complex at the restrictive temperature. SNR1 can establish direct contacts through the conserved repeat 2 region with the SET domain of the homeotic regulator Trithorax (TRX), and SNR1(E1) is partially defective for functional TRX association. As truncating mutations of INI1 are strongly correlated with aggressive cancers, our results support the view that SNR1, and specifically the repeat 2 region, has a critical role in mediating cell growth control functions of the metazoan SWI/SNF complexes.

Links

PubMed PMC140686

Keywords

Amino Acid Sequence; Animals; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Division/genetics; Chromatin/metabolism; Conserved Sequence; DNA-Binding Proteins/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/embryology; Drosophila melanogaster/genetics; Drosophila melanogaster/growth & development; Embryo, Nonmammalian; Eye/growth & development; Female; Gene Expression Regulation, Developmental; Genes, Lethal; Larva; Male; Molecular Sequence Data; Mutagenesis; Peripheral Nervous System/growth & development; Peripheral Nervous System/metabolism; Protein Subunits; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Veins/growth & development; Wing/blood supply; Wing/growth & development

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:Q24090

part_of

GO:0035060: brahma complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q24090

involved_in

GO:0008285: negative regulation of cell population proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q24090

enables

GO:0070984: SET domain binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0003862

F

Seeded From UniProt

complete


See also

References

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