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PMID:12481063

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Citation

Hsieh, MH and Goodman, HM (2002) Molecular characterization of a novel gene family encoding ACT domain repeat proteins in Arabidopsis. Plant Physiol. 130:1797-806

Abstract

In bacteria, the regulatory ACT domains serve as amino acid-binding sites in some feedback-regulated amino acid metabolic enzymes. We have identified a novel type of ACT domain-containing protein family in Arabidopsis whose members contain ACT domain repeats (the "ACR" protein family). There are at least eight ACR genes located on each of the five chromosomes in the Arabidopsis genome. Gene structure comparisons indicate that the ACR gene family may have arisen by gene duplications. Northern-blot analysis indicates that each member of the ACR gene family has a distinct expression pattern in various organs from 6-week-old Arabidopsis. Moreover, analyses of an ACR3 promoter-beta-glucuronidase (GUS) fusion in transgenic Arabidopsis revealed that the GUS activity formed a gradient in the developing leaves and sepals, whereas low or no GUS activity was detected in the basal regions. In 2-week-old Arabidopsis seedlings grown in tissue culture, the expression of the ACR gene family is differentially regulated by plant hormones, salt stress, cold stress, and light/dark treatment. The steady-state levels of ACR8 mRNA are dramatically increased by treatment with abscisic acid or salt. Levels of ACR3 and ACR4 mRNA are increased by treatment with benzyladenine. The amino acid sequences of Arabidopsis ACR proteins are most similar in the ACT domains to the bacterial sensor protein GlnD. The ACR proteins may function as novel regulatory or sensor proteins in plants.

Links

PubMed PMC166691 Online version:10.1104/pp.007484

Keywords

Adaptation, Physiological/drug effects; Adaptation, Physiological/genetics; Amino Acid Sequence; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Carrier Proteins/genetics; Carrier Proteins/metabolism; Chromosome Mapping; Cold Temperature; DNA, Complementary/chemistry; DNA, Complementary/genetics; Darkness; Gene Expression Regulation, Plant/drug effects; Glucuronidase/genetics; Glucuronidase/metabolism; Light; Molecular Sequence Data; Multigene Family/genetics; Plant Growth Regulators/pharmacology; Plants, Genetically Modified; Promoter Regions, Genetic/genetics; Receptors, Cell Surface/genetics; Receptors, Cell Surface/metabolism; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sodium Chloride/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:F4I0I7

located_in

GO:0005829: cytosol

ECO:0000250: sequence similarity evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:F4I0I7

acts_upstream_of_or_within

GO:0006521: regulation of cellular amino acid metabolic process

ECO:0000250: sequence similarity evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4I0I7

acts_upstream_of_or_within

GO:0009735: response to cytokinin

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ACR3

located_in

GO:0005829: cytosol

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

ARATH:ACR3

enables

GO:0016597: amino acid binding

ECO:0000250: sequence similarity evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:ACR7

located_in

GO:0005829: cytosol

ECO:0000250: sequence similarity evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:ACR7

enables

GO:0016597: amino acid binding

ECO:0000250: sequence similarity evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:ACR1

located_in

GO:0005634: nucleus

ECO:0000250: sequence similarity evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:ACR1

enables

GO:0016597: amino acid binding

ECO:0000250: sequence similarity evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:ACR8

acts_upstream_of_or_within

GO:0009737: response to abscisic acid

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ACR8

enables

GO:0016597: amino acid binding

ECO:0000250: sequence similarity evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:ACR6

enables

GO:0016597: amino acid binding

ECO:0000250: sequence similarity evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:ACR5

enables

GO:0016597: amino acid binding

ECO:0000250: sequence similarity evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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