PMID:12458200

Citation	Goto, H, Yasui, Y, Kawajiri, A, Nigg, EA, Terada, Y, Tatsuka, M, Nagata, K and Inagaki, M (2003) Aurora-B regulates the cleavage furrow-specific vimentin phosphorylation in the cytokinetic process. J. Biol. Chem. 278:8526-30
Abstract	Aurora-B is an evolutionally conserved protein kinase that regulates several mitotic events including cytokinesis. We previously demonstrated the possible existence of a protein kinase that phosphorylates at least Ser-72 on vimentin, the most widely expressed intermediate filament protein, in the cleavage furrow-specific manner. Here we showed that vimentin-Ser-72 phosphorylation occurred specifically at the border of the Aurora-B-localized area from anaphase to telophase. Expression of a dominant-negative mutant of Aurora-B led to a reduction of this vimentin-Ser-72 phosphorylation. In vitro analyses revealed that Aurora-B phosphorylates vimentin at approximately 2 mol phosphate/mol of substrate for 30 min and that this phosphorylation dramatically inhibits vimentin filament formation. We further identified eight Aurora-B phosphorylation sites, including Ser-72 on vimentin, and then constructed the mutant vimentin in which these identified sites are changed into Ala. Cells expressing this mutant formed an unusually long bridge-like intermediate filament structure between unseparated daughter cells. We then identified important phosphorylation sites for the bridge phenotype. Our findings indicate that Aurora-B regulates the cleavage furrow-specific vimentin phosphorylation and controls vimentin filament segregation in cytokinetic process.
Links	PubMed Online version:10.1074/jbc.M210892200
Keywords	Amino Acid Sequence; Animals; Cell Cycle; Mice; Molecular Sequence Data; Phosphorylation; Protein-Serine-Threonine Kinases/metabolism; Recombinant Proteins/metabolism; Vimentin/chemistry; Vimentin/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:AURKB		GO:0032467: positive regulation of cytokinesis	ECO:0000315:		P		Figure 2 a-d shows Aurora-B's role in inhibiting Vimentin and promoting cytokinesis	complete CACAO 2331
MOUSE:AURKB		GO:0006468: protein phosphorylation	ECO:0000314:		P		Fig. 2A shows Aurora-B phosphorylating Vimentin	complete

References

See Help:References for how to manage references in GONUTS.