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PMID:12442171

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Citation

Pellegrini, L, Yu, DS, Lo, T, Anand, S, Lee, M, Blundell, TL and Venkitaraman, AR (2002) Insights into DNA recombination from the structure of a RAD51-BRCA2 complex. Nature 420:287-93

Abstract

The breast cancer susceptibility protein BRCA2 controls the function of RAD51, a recombinase enzyme, in pathways for DNA repair by homologous recombination. We report here the structure of a complex between an evolutionarily conserved sequence in BRCA2 (the BRC repeat) and the RecA-homology domain of RAD51. The BRC repeat mimics a motif in RAD51 that serves as an interface for oligomerization between individual RAD51 monomers, thus enabling BRCA2 to control the assembly of the RAD51 nucleoprotein filament, which is essential for strand-pairing reactions during DNA recombination. The RAD51 oligomerization motif is highly conserved among RecA-like recombinases, highlighting a common evolutionary origin for the mechanism of nucleoprotein filament formation, mirrored in the BRC repeat. Cancer-associated mutations that affect the BRC repeat disrupt its predicted interaction with RAD51, yielding structural insight into mechanisms for cancer susceptibility.

Links

PubMed Online version:10.1038/nature01230

Keywords

Amino Acid Motifs; Amino Acid Sequence; Animals; BRCA2 Protein/chemistry; BRCA2 Protein/genetics; BRCA2 Protein/metabolism; Binding Sites; Breast Neoplasms/genetics; Conserved Sequence; Crystallography, X-Ray; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Genetic Predisposition to Disease; Humans; Hydrophobic and Hydrophilic Interactions; Macromolecular Substances; Models, Molecular; Molecular Sequence Data; Mutation/genetics; Protein Binding; Protein Structure, Tertiary; Rad51 Recombinase; Recombination, Genetic; Repetitive Sequences, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:BRCA2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q06609

F

Seeded From UniProt

complete

HUMAN:RAD51

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P51587

F

Seeded From UniProt

complete

HUMAN:RAD51

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:RAD51

involved_in

GO:0051260: protein homooligomerization

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q06609

P

Seeded From UniProt

complete

HUMAN:RAD51

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:RAD51

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q06609

F

Seeded From UniProt

complete


See also

References

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