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PMID:12438342
| Citation |
Clarke, SR, Harris, LG, Richards, RG and Foster, SJ (2002) Analysis of Ebh, a 1.1-megadalton cell wall-associated fibronectin-binding protein of Staphylococcus aureus. Infect. Immun. 70:6680-7 |
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| Abstract |
In order for Staphylococcus aureus to adhere to host extracellular matrix (ECM) substrates, it elicits a wide range of surface proteins. We have characterized a novel approximately 1.1-MDa protein in S. aureus, termed Ebh (for ECM-binding protein homologue), which has homology to other ECM-binding proteins. Ebh consists of several domains, including a large central region with 44 imperfect repeats of 126 amino acids. Expression analysis revealed ebh to be growth phase regulated and repressed by agr. A fragment of the central repeat region of Ebh was cloned, overexpressed, and used in ligand-binding studies to determine Ebh function. The recombinant protein was found to specifically bind human fibronectin. Ebh is produced during human infection since serum samples taken from patients with confirmed S. aureus infections were found to contain anti-Ebh antibodies. Localization studies revealed Ebh to be cell envelope associated and is proposed to form a specialized surface structure involved in cellular adhesion. |
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| Keywords |
Abscess; Adhesins, Bacterial; Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Wall/metabolism; Disease Models, Animal; Fibronectins/metabolism; Gene Expression Regulation, Bacterial; Humans; Mice; Mutation; Sequence Analysis, DNA; Staphylococcal Infections/microbiology; Staphylococcal Skin Infections/microbiology; Staphylococcus aureus/genetics; Staphylococcus aureus/metabolism; Staphylococcus aureus/pathogenicity; Transcription, Genetic; Virulence |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0030313: cell envelope |
ECO:0000314: |
C |
Fig 4. When the Ebh protein was removed from the cell wall by boiling it in a buffer there was no lines visible for the Western Blot. Meaning the protein is bound to the cell envelope. |
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See also
References
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