PMID:12435630

Citation	Dauwalder, B, Tsujimoto, S, Moss, J and Mattox, W (2002) The Drosophila takeout gene is regulated by the somatic sex-determination pathway and affects male courtship behavior. Genes Dev. 16:2879-92
Abstract	The Drosophila somatic sex-determination regulatory pathway has been well studied, but little is known about the target genes that it ultimately controls. In a differential screen for sex-specific transcripts expressed in fly heads, we identified a highly male-enriched transcript encoding Takeout, a protein related to a superfamily of factors that bind small lipophilic molecules. We show that sex-specific takeout transcripts derive from fat body tissue closely associated with the adult brain and are dependent on the sex determination genes doublesex (dsx) and fruitless (fru). The male-specific Doublesex and Fruitless proteins together activate Takeout expression, whereas the female-specific Doublesex protein represses takeout independently of Fru. When cells that normally express takeout are feminized by expression of the Transformer-F protein, male courtship behavior is dramatically reduced, suggesting that male identity in these cells is necessary for behavior. A loss-of-function mutation in the takeout gene reduces male courtship and synergizes with fruitless mutations, suggesting that takeout plays a redundant role with other fru-dependent factors involved in male mating behavior. Comparison of Takeout sequences to the Drosophila genome reveals a family of 20 related secreted factors. Expression analysis of a subset of these genes suggests that the takeout gene family encodes multiple factors with sex-specific functions.
Links	PubMed PMC187483 Online version:10.1101/gad.1010302
Keywords	Amino Acid Sequence; Animals; Animals, Genetically Modified; Behavior, Animal/physiology; Brain/physiology; Circadian Rhythm/genetics; Courtship; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Drosophila/physiology; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Fat Body/physiology; Female; Gene Expression Regulation, Developmental; Insect Proteins/genetics; Insect Proteins/metabolism; Male; Molecular Sequence Data; Mutation; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Ribonucleoproteins/genetics; Ribonucleoproteins/metabolism; Sequence Homology, Amino Acid; Sex Determination Processes; Transcription Factors/genetics; Transcription Factors/metabolism
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