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Dai, P, Shinagawa, T, Nomura, T, Harada, J, Kaul, SC, Wadhwa, R, Khan, MM, Akimaru, H, Sasaki, H, Colmenares, C and Ishii, S (2002) Ski is involved in transcriptional regulation by the repressor and full-length forms of Gli3. Genes Dev. 16:2843-8


Transcription factor Glioblastoma-3 (Gli3) is cleaved in the anterior region of the limb bud to generate its repressor form. In contrast, Sonic hedgehog (Shh) signaling from the posterior zone of polarizing activity blocks Gli3 processing and then induces the expression of Gli3 target genes, including Gli1. Here we report that the Ski corepressor binds to Gli3 and recruits the histone deacetylase complex. The Gli3-mediated repression was impaired by anti-Ski antibody and in Ski-deficient fibroblasts, and Shh-induced Gli1 gene transcription mediated by full-length Gli3 was inhibited by Ski. Furthermore, a Ski mutation enhanced the digit abnormalities caused by the Gli3 gene mutation. Thus, Ski plays an important role in pattern formation.


PubMed PMC187484 Online version:10.1101/gad.1017302


Acyltransferases/genetics; Acyltransferases/immunology; Acyltransferases/metabolism; Animals; Antibodies/pharmacology; Binding Sites; Cells, Cultured; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Drosophila Proteins; Fibroblasts/physiology; Gene Expression Regulation, Developmental/drug effects; Hedgehog Proteins; Histone Deacetylases/metabolism; Humans; Kruppel-Like Transcription Factors; Limb Deformities, Congenital/genetics; Mice; Mice, Mutant Strains; Mutation; Nerve Tissue Proteins; Oncogene Proteins/genetics; Oncogene Proteins/metabolism; Promoter Regions, Genetic; Rats; Repressor Proteins/genetics; Repressor Proteins/metabolism; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic; Two-Hybrid System Techniques



Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

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